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TitleThe entropic penalty of ordered water accounts for weaker binding of the antibiotic novobiocin to a resistant mutant of DNA gyrase: a thermodynamic and crystallographic study.
Journal, issue, pagesBiochemistry, Vol. 36, Page 9663-9673, Year 1997
Publish dateMay 15, 1997 (structure data deposition date)
AuthorsHoldgate, G.A. / Tunnicliffe, A. / Ward, W.H. / Weston, S.A. / Rosenbrock, G. / Barth, P.T. / Taylor, I.W. / Pauptit, R.A. / Timms, D.
External linksBiochemistry / PubMed:9245398
MethodsX-ray diffraction
Resolution2.3 Å
Structure data

PDB-1aj6:
NOVOBIOCIN-RESISTANT MUTANT (R136H) OF THE N-TERMINAL 24 KDA FRAGMENT OF DNA GYRASE B COMPLEXED WITH NOVOBIOCIN AT 2.3 ANGSTROMS RESOLUTION
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

Chemicals

ChemComp-NOV:
NOVOBIOCIN / antibiotic*YM

ChemComp-HOH:
WATER

Source
  • escherichia coli (E. coli)
KeywordsTOPOISOMERASE / GYRASE / NOVOBIOCIN / ANTIBIOTIC / RESISTANT MUTANT

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