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TitleUniversal pipeline for high-resolution GPCR structure determination.
Journal, issue, pagesNat Struct Mol Biol, Year 2026
Publish dateAug 28, 2026
AuthorsAsato Kojima / Kouki Kawakami / Naoya Kobayashi / Kazuhiro Kobayashi / Toshiki E Matsui / Kohei Uemoto / Yuzhong Gu / Tomohiro J Narita / Mai Kugawa / Masahiro Fukuda / Hideaki E Kato /
PubMed AbstractG protein-coupled receptors (GPCRs) regulate human physiology and are major drug targets. Although cryo-electron microscopy has accelerated GPCR structural biology, inactive-state structures remain ...G protein-coupled receptors (GPCRs) regulate human physiology and are major drug targets. Although cryo-electron microscopy has accelerated GPCR structural biology, inactive-state structures remain difficult because current fusion-based strategies often require extensive experimental screening to identify rigid constructs suitable for high-resolution reconstruction. Here we introduce a universal pipeline that integrates an in silico fusion construct screening program, NOAH (nonexperimental, artificial-intelligence-assisted, high-throughput construct screening for structural analysis), with a de novo designed fusion protein, ARK1 (artificially designed fiducial marker). NOAH enabled structure determination of vasopressin V2 receptor bound to the antagonist tolvaptan or partial agonist OPC51803 and bradykinin B2 receptor bound to the antagonist icatibant, revealing receptor activation and inhibition mechanisms. Coupling NOAH to ARK1 improved the V2 receptor-tolvaptan map and enabled high-resolution structures of lysophosphatidic acid receptor 2 bound to Ki16425 and free fatty acid receptor 2 bound to GLPG0974. NOAH-ARK1 minimizes trial-and-error construct optimization and provides a broadly applicable route for GPCR structural analysis and drug discovery.
External linksNat Struct Mol Biol / PubMed:42665663
MethodsEM (single particle)
Resolution2.93 Å
Structure data

67995

21ty

EMDB-67995, PDB-21ty:
Cryo-EM structure of Free fatty acid receptor 2 (FFA2)-ARK1 with GLPG0974
Method: EM (single particle) / Resolution: 2.93 Å

Chemicals

PDB-1lyd:
CRYSTAL STRUCTURE OF T4-LYSOZYME GENERATED FROM SYNTHETIC CODING DNA EXPRESSED IN ESCHERICHIA COLI

ChemComp-HOH:
WATER

Source
  • homo sapiens (human)
KeywordsMEMBRANE PROTEIN / GPCR / FFA2 / Free fatty acid receptor 2 / GLPG0974 / ARK1

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