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Structure paper

TitleIn situ structures of plant photosystem supercomplexes.
Journal, issue, pagesNature, Year 2026
Publish dateJul 29, 2026
AuthorsJiao Li / Eduard Elias / Kai Zhang / Roberta Croce / Jiapeng Zhu /
PubMed AbstractPhotosynthesis sustains life on Earth by converting light to chemical energy through the coordinated action of photosystem I (PSI) and photosystem II (PSII) within thylakoid membranes. Although ...Photosynthesis sustains life on Earth by converting light to chemical energy through the coordinated action of photosystem I (PSI) and photosystem II (PSII) within thylakoid membranes. Although structures of isolated photosystems are available, their native organization in chloroplasts remains unknown. Here, using in situ cryo-electron microscopy, we directly imaged Oryza sativa (rice) chloroplasts and determined structures of photosystem supercomplexes in their native membrane environment. We resolved a CSML-type PSII-light harvesting complex II (LHCII) supercomplex, including four LHCII antenna trimers that were not retained in purified preparations. Excitation energy transfer calculations based on this architecture closely reproduce in vivo measurements, indicating its physiological relevance. We also resolved asymmetric PSII-LHCII dimers, including side-by-side, trans-lumenal and trans-stromal architectures, and higher-order assemblies of trimers and tetramers. On the basis of these observations, we propose that PSII forms a trans-lumenal and trans-stromal 'skeleton' that shapes thylakoid morphology and supports grana stacking. In addition, we obtained high-resolution structures of PSI-LHCI-LHCII and PSI-LHCI supercomplexes. Together, these structures reveal extensive networks of lipids, pigments and cofactors, providing the first molecular framework for understanding how the native architecture of plant photosystem supports the exceptional photon-to-electron efficiency of photosynthesis.
External linksNature / PubMed:42527593
MethodsEM (single particle)
Resolution2.38 - 2.96 Å
Structure data

66925

9xj1

EMDB-66925, PDB-9xj1:
In situ structure of the PSI-LHCI supercomplex from Oryza sativa
Method: EM (single particle) / Resolution: 2.38 Å

66932

9xj9

EMDB-66932, PDB-9xj9:
In situ structure of the PSI-LHCI-LHCII supercomplex from Oryza sativa
Method: EM (single particle) / Resolution: 2.96 Å

Chemicals

ChemComp-CHL:
CHLOROPHYLL B

ChemComp-CLA:
CHLOROPHYLL A

ChemComp-LUT:
(3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL

ChemComp-XAT:
(3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL

ChemComp-BCR:
BETA-CAROTENE

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM

ChemComp-MGE:
(1S)-2-(ALPHA-L-ALLOPYRANOSYLOXY)-1-[(TRIDECANOYLOXY)METHYL]ETHYL PALMITATE

ChemComp-PQN:
PHYLLOQUINONE

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-CL0:
CHLOROPHYLL A ISOMER

ChemComp-DGD:
DIGALACTOSYL DIACYL GLYCEROL (DGDG)

Source
  • Oryza sativa subsp. japonica (Japanese rice)
  • oryza sativa japonica group (Japanese rice)
KeywordsPHOTOSYNTHESIS / Photosystem I / PSI-LHCI / in situ / Oryza sativa / chloroplast / PSI-LHCI-LHCII

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