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Structure paper

TitleDiffraction-quality, ultraflexible protein single crystals engineered with DNA.
Journal, issue, pagesSci Adv, Vol. 12, Page eaeh2948-eaeh2948, Year 2026
Publish dateJan 29, 2025 (structure data deposition date)
AuthorsHan, Z. / Mirkin, C.A.
External linksSci Adv / PubMed:42525757
MethodsX-ray diffraction
Resolution1.42 - 3.15 Å
Structure data

PDB-9n2u:
Glutarate L-2-hydroxylase N187C mutant-5'-Mal-C6-GC DNA conjugate
Method: X-RAY DIFFRACTION / Resolution: 2.06 Å

PDB-9n33:
Glutarate L-2-hydroxylase N187C mutant-5'-Mal-C6-AGCT DNA conjugate
Method: X-RAY DIFFRACTION / Resolution: 2.61 Å

PDB-9n34:
Glutarate L-2-hydroxylase N187C mutant-5'-Mal-C6-TTTT DNA conjugate
Method: X-RAY DIFFRACTION / Resolution: 2.86 Å

PDB-9n53:
Glutarate L-2-hydroxylase K270C mutant-5'-Mal-C2-AAATTT DNA conjugate in P 4 21 2 space group
Method: X-RAY DIFFRACTION / Resolution: 1.65 Å

PDB-9n56:
Glutarate L-2-hydroxylase K270C mutant-5'-Mal-C2-AGCT DNA conjugate
Method: X-RAY DIFFRACTION / Resolution: 2.25 Å

PDB-9n57:
Glutarate L-2-hydroxylase K270C mutant-5'-Mal-C2-AAATTT DNA conjugate in I422 space group
Method: X-RAY DIFFRACTION / Resolution: 1.64 Å

PDB-9n5s:
Glutarate L-2-hydroxylase K270C mutant-5'-Mal-C2-GC DNA conjugate
Method: X-RAY DIFFRACTION / Resolution: 2.73 Å

PDB-9n5w:
Glutarate L-2-hydroxylase Q184C mutant-5'-Mal-C6-GC DNA conjugate
Method: X-RAY DIFFRACTION / Resolution: 2.06 Å

PDB-9n60:
Glutarate L-2-hydroxylase Q184C mutant-5'-Mal-C6-AAATTT DNA conjugate at 2.47 Angstrom resolution
Method: X-RAY DIFFRACTION / Resolution: 2.47 Å

PDB-9n6s:
Glutarate L-2-hydroxylase Q184C mutant-5'-Mal-C6-AGCT DNA conjugate at 2.47 Angstrom resolution
Method: X-RAY DIFFRACTION / Resolution: 2.47 Å

PDB-9n7c:
Glutarate L-2-hydroxylase Q184C mutant-5'-Mal-C6-TTTT DNA conjugate at 2.02 Angstrom resolution
Method: X-RAY DIFFRACTION / Resolution: 2.023 Å

PDB-9n7h:
Glutarate L-2-hydroxylase Q184C mutant-5'-Mal-C6-AGCT DNA conjugate at 2.37 Angstrom resolution
Method: X-RAY DIFFRACTION / Resolution: 2.37 Å

PDB-9n7i:
Glutarate L-2-hydroxylase Q184C mutant-5'-Mal-C6-AAATTT DNA conjugate at 3.15 Angstrom resolution
Method: X-RAY DIFFRACTION / Resolution: 3.15 Å

PDB-9n7j:
Glutarate L-2-hydroxylase Q184C mutant-5'-Mal-C6-TTTT DNA conjugate at 3.12 Angstrom resolution
Method: X-RAY DIFFRACTION / Resolution: 3.121 Å

PDB-9n7l:
Glutarate L-2-hydroxylase Q184C mutant-5'-Mal-C6-AGCT DNA conjugate at 2.86 Angstrom resolution
Method: X-RAY DIFFRACTION / Resolution: 2.86 Å

PDB-9n7n:
Glutarate L-2-hydroxylase Q184C mutant-5'-Mal-C6-AGCT DNA conjugate at 1.82 Angstrom resolution
Method: X-RAY DIFFRACTION / Resolution: 1.82 Å

PDB-9n7p:
Glutarate L-2-hydroxylase Q184C mutant-5'-Mal-C2-AGCT DNA conjugate at 1.89 Angstrom resolution
Method: X-RAY DIFFRACTION / Resolution: 1.891 Å

PDB-9n7u:
Glutarate L-2-hydroxylase Q184C mutant-5'-Mal-C2-AGCT DNA conjugate at 2.17 Angstrom resolution
Method: X-RAY DIFFRACTION / Resolution: 2.17 Å

PDB-9n7x:
Glutarate L-2-hydroxylase Q184C mutant-5'-Mal-C2-AGCT DNA conjugate at 2.61 Angstrom resolution
Method: X-RAY DIFFRACTION / Resolution: 2.61 Å

PDB-9n8c:
Glutarate L-2-hydroxylase Q184C mutant-5'-Mal-C2-GTAC DNA conjugate
Method: X-RAY DIFFRACTION / Resolution: 1.61 Å

PDB-9n8d:
Glutarate L-2-hydroxylase N182A, Q184C mutant-5'-Mal-C2-AGCT DNA conjugate
Method: X-RAY DIFFRACTION / Resolution: 1.84 Å

PDB-9n8k:
Glutarate L-2-hydroxylase Q184C, K270A mutant-5'-Mal-C2-AGCT DNA conjugate
Method: X-RAY DIFFRACTION / Resolution: 1.77 Å

PDB-9n8l:
Glutarate L-2-hydroxylase N182A, Q184C, K270A mutant-5'-Mal-C2-AGCT DNA conjugate
Method: X-RAY DIFFRACTION / Resolution: 1.42 Å

PDB-9n8o:
Glutarate L-2-hydroxylase Q184C mutant-5'-Mal-C2-AGGCCT DNA conjugate
Method: X-RAY DIFFRACTION / Resolution: 2.05 Å

PDB-9n8s:
Glutarate L-2-hydroxylase Q184C mutant-5'-Mal-C2-AGCT DNA conjugate at 2.03 Angstrom resolution
Method: X-RAY DIFFRACTION / Resolution: 2.03 Å

PDB-9n8t:
Glutarate L-2-hydroxylase Q184C mutant-5'-Mal-C2-AGCT DNA conjugate at 2.01 Angstrom resolution
Method: X-RAY DIFFRACTION / Resolution: 2.01 Å

PDB-9n8u:
Glutarate L-2-hydroxylase Q184C mutant-5'-Mal-C2-AGCT DNA conjugate at 1.74 Angstrom resolution
Method: X-RAY DIFFRACTION / Resolution: 1.74 Å

PDB-9n8v:
Glutarate L-2-hydroxylase Q184C mutant-5'-Mal-C2-AGCT DNA conjugate at 1.78 Angstrom resolution
Method: X-RAY DIFFRACTION / Resolution: 1.78 Å

Chemicals

PDB-1bvo:
DORSAL HOMOLOGUE GAMBIF1 BOUND TO DNA

ChemComp-FE2:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-NEN:
1-ETHYL-PYRROLIDINE-2,5-DIONE

ChemComp-SO4:
SULFATE ION

ChemComp-PO4:
PHOSPHATE ION

PDB-1bwd:
INOSAMINE-PHOSPHATE AMIDINOTRANSFERASE STRB1 FROM STREPTOMYCES GRISEUS

ChemComp-GOL:
GLYCEROL

PDB-1bwe:
ARTIFICIAL FE8S8 FERREDOXIN: THE D13C VARIANT OF BACILLUS SCHLEGELII FE7S8 FERREDOXIN

ChemComp-NH4:
AMMONIUM ION

ChemComp-CL:
Unknown entry

ChemComp-MG:
Unknown entry

Source
  • escherichia coli (E. coli)
  • synthetic construct (others)
KeywordsMETAL BINDING PROTEIN / Oxygenase / Hydroxylase / Metal binding

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