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TitleDe novo design of orthogonal far-red, orange, and green fluorophore-binding proteins for multiplexed imaging.
Journal, issue, pagesScience, Page eaeb0822-eaeb0822, Year 2026
Publish dateMar 5, 2026 (structure data deposition date)
AuthorsTran, L. / Klein, S. / Juergens, D. / Sharma, S. / Decarreau, J. / Lee, G.R. / Wang, Y. / Chen, W. / Bera, A.K. / Kang, A. ...Tran, L. / Klein, S. / Juergens, D. / Sharma, S. / Decarreau, J. / Lee, G.R. / Wang, Y. / Chen, W. / Bera, A.K. / Kang, A. / Woods, J. / Joyce, E. / Vafeados, D.K. / Roullier, N. / Li, X. / Liu, B. / Bo, Y. / Muratspahic, E. / Brown, T.A. / Grimm, J.B. / Patel, R. / Lavis, L.D. / Mahamid, J. / An, L. / Baker, D.
External linksScience / PubMed:42461986
MethodsX-ray diffraction
Resolution1.44 - 2.38 Å
Structure data

PDB-11mq:
Crystal Structure of Fluorophore-binding Protein NovoTag657-holo
Method: X-RAY DIFFRACTION / Resolution: 2.38 Å

PDB-9pvl:
Crystal Structure of Fluorophore-binding Protein JF657-bp
Method: X-RAY DIFFRACTION / Resolution: 1.44 Å

Chemicals

ChemComp-SO4:
SULFATE ION

ChemComp-ZN:
Unknown entry

PDB-1dmm:
CRYSTAL STRUCTURES OF MUTANT ENZYMES Y57F OF KETOSTEROID ISOMERASE FROM PSEUDOMONAS PUTIDA BIOTYPE B

ChemComp-HOH:
WATER

ChemComp-PE4:
2-{2-[2-(2-{2-[2-(2-ETHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHOXY)-ETHOXY]-ETHOXY}-ETHANOL / precipitant*YM

ChemComp-CA:
Unknown entry

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

Source
  • synthetic construct (others)
KeywordsDE NOVO PROTEIN / de novo designed protein / Small molecule binder / Fluorophore-binding / Multiplex Imaging

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