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TitleEasyGrid: a versatile platform for automated cryo-EM sample preparation and quality control.
Journal, issue, pagesNat. Methods, Year 2026
Publish dateFeb 20, 2026
AuthorsOlivier Gemin / Victor Armijo / Léa Lecomte / Michael Hons / Thibault Deckers / Caroline Bissardon / Christopher Rossi / Kévin Lauzier / Robert Janocha / Franck Felisaz / Jérémy Sinoir / Romain Linares / Anastasiia Babenko / Kirill Kovalev / Irina Prokhorova / Iskander Khusainov / Claudia Schreiner / Olga Kolesnikova / Veijo T Salo / Sarah Schneider / Matthew W Bowler / Georg Wolff / Wojciech P Galej / Julia Mahamid / Christoph W Müller / Kristina Djinovic Carugo / Sebastian Eustermann / Simone Mattei / Florent Cipriani / Gergely Papp /
PubMed AbstractOptimized sample preparation is essential for imaging biological macromolecules in their native state using single-particle cryo-electron microscopy (cryo-EM) or in situ cryo-electron tomography ...Optimized sample preparation is essential for imaging biological macromolecules in their native state using single-particle cryo-electron microscopy (cryo-EM) or in situ cryo-electron tomography (cryo-ET). Here we present EasyGrid, a modular, automated platform designed to streamline and standardize cryo-EM/ET sample preparation. EasyGrid integrates in-line plasma treatment of the sample support, microfluidic dispensing, blot-less sample spreading, jet-based vitrification and grid quality control via light interferometry. We demonstrate its effectiveness by preparing grids for multiple purified macromolecular complexes and resolving their structures with cryo-EM. Additionally, EasyGrid achieves improved vitrification of large mammalian cells compared to conventional plunge-freezing. By enabling systematic and high-throughput optimization, EasyGrid provides a robust and time-saving solution for both structural and cellular cryo-EM applications.
External linksNat. Methods / PubMed:42337080
MethodsEM (single particle) / EM (subtomogram averaging)
Resolution1.94 - 6.8 Å
Structure data

19434

8rq5

EMDB-19434, PDB-8rq5:
Cryo-EM structure of the light-driven sodium-pumping rhodopsin KR2
Method: EM (single particle) / Resolution: 2.32 Å

EMDB-19880: Cryo-EM structure of human apoferritin (grid prepared with EasyGrid technology)
Method: EM (single particle) / Resolution: 1.94 Å

EMDB-56607: CryoEM structure of guanidinase from Nitrospira inopinata
Method: EM (single particle) / Resolution: 3.1 Å

EMDB-56821: C.t. INO80 chromatin remodeler bound to nucleosome
Method: EM (single particle) / Resolution: 4.93 Å

EMDB-56855: Cryo-EM structure of yeast ribosome solved with EasyGrid
Method: EM (single particle) / Resolution: 2.43 Å


EMDB Unreleased entry

EMDB-56861: In situ ribosome from HeLa cells
Method: EM (subtomogram averaging) / Resolution: 6.8 Å

Chemicals

ChemComp-OLC:
(2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate

ChemComp-LFA:
EICOSANE

ChemComp-RET:
RETINAL

ChemComp-NA:
Unknown entry

ChemComp-OLA:
OLEIC ACID

ChemComp-HOH:
WATER

Source
  • dokdonia eikasta (bacteria)
  • Homo sapiens (human)
  • Candidatus Nitrospira inopinata (bacteria)
  • Thermochaetoides thermophila (fungus)
  • Schizosaccharomyces pombe 972h- (yeast)
KeywordsMEMBRANE PROTEIN / retinal / ion transport / sodium / rhodopsin / photocycle / pentamer

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