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TitleStructural basis of regioselective double halogenation of the beta-carboline tryptoline by the single-component halogenase AetF.
Journal, issue, pagesActa Crystallogr D Struct Biol, Vol. 82, Page 836-844, Year 2026
Publish dateMar 26, 2026 (structure data deposition date)
AuthorsBork, S. / Horstmeier, H.J. / Scharkowski, B. / Niemann, H.H.
External linksActa Crystallogr D Struct Biol / PubMed:42329691
MethodsX-ray diffraction
Resolution1.78 - 1.87 Å
Structure data

PDB-29oi:
AetF, a single-component flavin-dependent tryptophan halogenase, in complex with 8-bromotryptoline
Method: X-RAY DIFFRACTION / Resolution: 1.78 Å

PDB-9h6z:
AetF, a single-component flavin-dependent tryptophan halogenase, in complex with tryptoline
Method: X-RAY DIFFRACTION / Resolution: 1.87 Å

Chemicals

ChemComp-FAD:
FLAVIN-ADENINE DINUCLEOTIDE / FAD*YM

PDB-1j4b:
Recombinant Mouse-Muscle Adenylosuccinate Synthetase

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-CL:
Unknown entry

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-HOH:
WATER

ChemComp-WYH:
2,3,4,9-tetrahydro-1~{H}-pyrido[3,4-b]indole

Source
  • aetokthonos hydrillicola thurmond2011 (bacteria)
KeywordsFLAVOPROTEIN / single-component halogenase / tryptophan halogenase / non-native substrate / unnatural substrate

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