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TitleNear-complete cryo-EM structure of the Klebsiella pneumoniae podophage RAN69 reveals tail fiber-spike interface and a divergent pre-ejectosome.
Journal, issue, pagesStructure, Year 2026
Publish dateJun 18, 2026
AuthorsZhiyang Ruan / Hongli Hu / Aohan Wang / Qianqian Shao / Xiangyun Li / Linlin Xie / Zhuo Sun / Jiaxin Yu / Qianglin Fang /
PubMed AbstractCarbapenem-resistant Klebsiella pneumoniae is a critical-priority pathogen, underscoring the need for alternatives to antibiotics. Bacteriophages are promising agents, yet high-resolution structures ...Carbapenem-resistant Klebsiella pneumoniae is a critical-priority pathogen, underscoring the need for alternatives to antibiotics. Bacteriophages are promising agents, yet high-resolution structures of K. pneumoniae phages are scarce. Here, we report near-complete cryo-electron microscopic reconstructions of the K. pneumoniae podophage RAN69 at 3.0-3.4 Å resolution, enabling atomic models for 12 structural components. Complete in situ structures of the long tail fiber (gp9 trimer) and distal tail spike (gp1 trimer) are resolved, revealing a previously unknown binding interface in which the gp1 N-terminal arm wedges between spike-binding domains of gp9. The pre-ejectosome forms a conserved double-layered assembly (gp10 tetramer over gp11 octamer), tethered to the portal by an octameric gp12 that inserts long helices into the portal barrel, with gp20 reinforcing gp11 interfaces. Divergent folds in gp10 and a lysozyme-like gp11 peripheral domain suggest adaptation to host envelopes. These structures advance our understanding of podophage assembly.
External linksStructure / PubMed:42314677
MethodsEM (single particle)
Resolution3.0 - 3.8 Å
Structure data

69207

23sf

EMDB-69207, PDB-23sf:
Cryo-EM structure of icosahedrally averaged bacteriophage RAN69 capsid
Method: EM (single particle) / Resolution: 3.4 Å

69208

23sg

EMDB-69208, PDB-23sg:
The composite Cryo-EM structure of bacteriophage RAN69 pre-ejectosome-portal complex
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-69209: The consensus Cryo-EM structure of bacteriophage RAN69 pre-ejectosome-portal complex
Method: EM (single particle) / Resolution: 3.1 Å

EMDB-69210: Local refinement of the portal region within the bacteriophage RAN69 pre-ejectosome-portal complex
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-69211: Local refinement of the pre-ejectosome region within the bacteriophage RAN69 pre-ejectosome-portal complex
Method: EM (single particle) / Resolution: 3.0 Å

69212

23sh

EMDB-69212, PDB-23sh:
The composite Cryo-EM structure of the tail region of bacteriophage RAN69
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-69213: The consensus Cryo-EM structure of the tail region of bacteriophage RAN69
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-69214: Local refinement of bacteriophage RAN69 portal-tail complex
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-69215: Local refinement of bacteriophage RAN69 tail spike
Method: EM (single particle) / Resolution: 3.0 Å

Source
  • klebsiella phage ran69 (virus)
KeywordsVIRUS / bacteriophage / RAN69 / capsid / pre-ejectosome-portal complex / tail

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