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TitleAtomic resolution cryo-EM at 200 keV.
Journal, issue, pagesIUCrJ, Vol. 13, Issue Pt 4, Page 343-353, Year 2026
Publish dateJul 1, 2026
AuthorsRadostin Danev / Haruaki Yanagisawa / Keitaro Yamashita / Fabian Eisenstein / Masahide Kikkawa /
PubMed AbstractAtomic resolution in cryo-electron microscopy was first demonstrated six years ago. This was accomplished using 300 kV electron microscopes equipped with new hardware that provided narrower energy ...Atomic resolution in cryo-electron microscopy was first demonstrated six years ago. This was accomplished using 300 kV electron microscopes equipped with new hardware that provided narrower energy spread, aberration correction and energy filtering. Here, we report the achievement of 1.24 Å atomic resolution on an upgraded 200 kV electron microscope featuring a cold field emission gun, a high-resolution objective lens polepiece and an energy filter. These components transform the instrument into a cost-effective single-particle cryo-EM platform with performance comparable to that of significantly more expensive 300 kV systems. The microscope can also be operated at 100 kV and by using a high-speed hybrid-pixel detector we were able to reach sub-2 Å resolution.
External linksIUCrJ / PubMed:42268577 / PubMed Central
MethodsEM (single particle)
Resolution1.24 - 1.91 Å
Structure data

EMDB-68204: 100 kV cryo-EM structure of apoferritin at 1.91 A with DECTRIS SINGLA detector on CRYO ARM 200 II
Method: EM (single particle) / Resolution: 1.91 Å

68251

22fx

EMDB-68251, PDB-22fx:
Cryo-EM structure of mouse heavy-chain apoferritin at 1.24 A on CRYO ARM 200 II
Method: EM (single particle) / Resolution: 1.24 Å

Chemicals

ChemComp-FE:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-HOH:
WATER

Source
  • mus musculus (house mouse)
KeywordsMETAL BINDING PROTEIN / apoferritin

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