Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structure and engineering of the large serine recombinase Bxb1 for gene integration.
Journal, issue, pages	Mol Cell, Vol. 86, Issue 12, Page 2247-22262.e7, Year 2026
Publish date	Jun 18, 2026
Authors	Teppei Soma / Masahiro Hiraizumi / Christopher W Fell / Dario Tagliaferri / Jason Lequyer / Sae Okazaki / Yukari Isayama / Kazuki Kato / Sworaj Sapkota / Harsh Ramani / Benjamin Arya / Cian Schmitt-Ulms / Keitaro Yamashita / Jonathan S Gootenberg / Omar O Abudayyeh / Hiroshi Nishimasu /
PubMed Abstract	The large serine recombinase Bxb1 catalyzes recombination between DNA molecules containing compatible attP and attB sequences, offering broad applications in genome engineering and gene therapies. ...The large serine recombinase Bxb1 catalyzes recombination between DNA molecules containing compatible attP and attB sequences, offering broad applications in genome engineering and gene therapies. Here, we present cryo-electron microscopy structures of the Bxb1-attP-attB synaptic complex in four distinct functional states during its recombination cycle. Notably, the Bxb1 complex structures in the pre-, mid-, and post-strand-exchange states explain how the attP- and attB-bound Bxb1 dimers are assembled into a tetrameric synaptic complex and how an approximately 180° rotation occurs between the left and right dimers after DNA cleavage, thereby enabling DNA strand exchange and religation. Furthermore, we engineered Bxb1 variants with altered DNA preferences and enhanced recombination activity, which improved programmable gene integration in human cells. Overall, our findings advance the mechanistic understanding of large serine recombinases and provide a structural framework for future engineering of Bxb1-mediated genome integration technologies.
External links	Mol Cell / PubMed:42259299
Methods	EM (single particle)
Resolution	3.08 - 4.08 Å
Structure data	60887 9iu2 EMDB-60887, PDB-9iu2: Cryo-EM structure of the large serine recombinase Bxb1 in complex with attP and attB (GT/TT CDN) in the pre-strand exchange state Method: EM (single particle) / Resolution: 3.22 Å 60888 9iu3 EMDB-60888, PDB-9iu3: Cryo-EM structure of the large serine recombinase Bxb1 in complex with attP and attB (GT/TT CDN) in the post-strand exchange state Method: EM (single particle) / Resolution: 3.97 Å 60889 9iu4 EMDB-60889, PDB-9iu4: Cryo-EM structure of the large serine recombinase Bxb1 in complex with attP and attB (CA/CA CDN) in the pre-strand exchange state Method: EM (single particle) / Resolution: 3.58 Å 60890 9iu5 EMDB-60890, PDB-9iu5: Cryo-EM structure of the large serine recombinase Bxb1 in complex with attP and attB (CA/CA CDN) in the post-strand exchange state Method: EM (single particle) / Resolution: 3.63 Å 60891 9iu6 EMDB-60891, PDB-9iu6: Cryo-EM structure of the large serine recombinase Bxb1 in complex with attP and attB (CA/CA CDN) in the intermediate-strand exchange state 1 Method: EM (single particle) / Resolution: 3.97 Å 60892 9iu7 EMDB-60892, PDB-9iu7: Cryo-EM structure of the large serine recombinase Bxb1 in complex with attP and attB (CA/CA CDN) in the intermediate-strand exchange state 2 Method: EM (single particle) / Resolution: 4.08 Å 60893 9iu8 EMDB-60893, PDB-9iu8: Cryo-EM structure of the large serine recombinase Bxb1 in complex with attP and attB (GT/TT CDN) in the pre-strand exchange state (attP-L) Method: EM (single particle) / Resolution: 3.21 Å 60894 9iu9 EMDB-60894, PDB-9iu9: Cryo-EM structure of the large serine recombinase Bxb1 in complex with attP and attB (GT/TT CDN) in the pre-strand exchange state (attP-R) Method: EM (single particle) / Resolution: 3.21 Å 60895 9iua EMDB-60895, PDB-9iua: Cryo-EM structure of the large serine recombinase Bxb1 in complex with attP and attB (GT/TT CDN) in the pre-strand exchange state (attB-L) Method: EM (single particle) / Resolution: 3.78 Å 60896 9iub EMDB-60896, PDB-9iub: Cryo-EM structure of the large serine recombinase Bxb1 in complex with attP and attB (GT/TT CDN) in the pre-strand exchange state (attB-R) Method: EM (single particle) / Resolution: 3.08 Å
Chemicals	ChemComp-ZN: Unknown entry
Source	mycolicibacterium smegmatis (bacteria) mycobacterium phage bxb1 (virus)
Keywords	DNA BINDING PROTEIN / large serine recombinase