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TitleMechanism for the initiation of co-transcriptional pre-60 S assembly.
Journal, issue, pagesBiorxiv, Year 2026
Publish dateFeb 13, 2026 (structure data deposition date)
AuthorsRafal Piwowarczyk / Sebastian Klinge
PubMed AbstractEukaryotic ribosomal large subunit (60 ) assembly requires an internal transcribed spacer 2 (ITS2) to license both nucleolar and nuclear pre-60 assembly intermediates. The underlying molecular ...Eukaryotic ribosomal large subunit (60 ) assembly requires an internal transcribed spacer 2 (ITS2) to license both nucleolar and nuclear pre-60 assembly intermediates. The underlying molecular mechanisms responsible for nucleation of pre-60 assembly, quality control, and installation of ITS2 during co-transcriptional stages remain unknown. Here we report the earliest co-transcriptional assembly intermediates of the eukaryotic 60 subunits. Together with biochemical assays, our data reveal the architecture of co-transcriptional pre-60 assembly initiation and progression, as well as the molecular logic of an assembly checkpoint. This study highlights an evolutionary solution by which complex RNA folding processes can be parallelized and integrated via biological AND-gating.
External linksBiorxiv / PubMed:42239465 / PubMed Central
MethodsEM (single particle)
Resolution2.74 - 2.8 Å
Structure data

PDB-10zy:
Yeast co-transcriptional pre-60S assembly intermediate Pwp1 RNP
Method: ELECTRON MICROSCOPY / Resolution: 2.74 Å

PDB-10zz:
Yeast co-transcriptional pre-60S assembly intermediate Pwp1 RNP*
Method: ELECTRON MICROSCOPY / Resolution: 2.8 Å

PDB-11aa:
Yeast co-transcriptional pre-60S assembly intermediate Nop12 RNP
Method: ELECTRON MICROSCOPY / Resolution: 2.78 Å

Chemicals

ChemComp-MG:
Unknown entry

Source
  • saccharomyces cerevisiae s288c (yeast)
KeywordsRIBOSOME / ribosome biogenesis / co-transcriptional assembly / RNA-binding protein / nucleolar RNP

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