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TitleComputational engineering of the polyester hydrolase PHL7 for efficient poly(ethylene terephthalate) degradation in biocatalytic recycling processes.
Journal, issue, pagesNat Commun, Vol. 17, Year 2026
Publish dateMar 25, 2025 (structure data deposition date)
AuthorsBlazquez-Sanchez, P. / Gunkel, J. / Useini, A. / Zlobin, A. / Zakary, J.D. / Scholer, A. / Graefe, N. / Engelberger, F. / Cantanhede, F. / Frank, R. ...Blazquez-Sanchez, P. / Gunkel, J. / Useini, A. / Zlobin, A. / Zakary, J.D. / Scholer, A. / Graefe, N. / Engelberger, F. / Cantanhede, F. / Frank, R. / Zhao, Z. / Zarei, A. / Butenschon, E. / Matysik, J. / Zimmermann, W. / Strater, N. / Sonnendecker, C. / Kunze, G.
External linksNat Commun / PubMed:42140896
MethodsX-ray diffraction
Resolution1.1 - 1.59 Å
Structure data

PDB-9qnm:
Polyester Hydrolase Leipzig 7 (PHL7) variant R2M2
Method: X-RAY DIFFRACTION / Resolution: 1.12 Å

PDB-9qt8:
Polyester Hydrolase Leipzig 7 (PHL7) variant R2M2-P155G
Method: X-RAY DIFFRACTION / Resolution: 1.21 Å

PDB-9qv8:
Polyester Hydrolase Leipzig 7 (PHL7) variant R2M2-A186S
Method: X-RAY DIFFRACTION / Resolution: 1.25 Å

PDB-9qva:
Polyester Hydrolase Leipzig 7 (PHL7) variant R2M2-P155G-A186S
Method: X-RAY DIFFRACTION / Resolution: 1.59 Å

PDB-9qya:
Polyester Hydrolase Leipzig 7 (PHL7) variant R4M6
Method: X-RAY DIFFRACTION / Resolution: 1.53 Å

PDB-9qyb:
Polyester Hydrolase Leipzig 7 (PHL7) variant R4M10
Method: X-RAY DIFFRACTION / Resolution: 1.1 Å

PDB-9qyc:
Polyester Hydrolase Leipzig 7 (PHL7) variant R4M12
Method: X-RAY DIFFRACTION / Resolution: 1.16 Å

Chemicals

ChemComp-CIT:
CITRIC ACID

ChemComp-HOH:
WATER

ChemComp-CL:
Unknown entry

Source
  • compost metagenome (others)
KeywordsHYDROLASE / PET hydrolase / enzymatic plastic degradation / enzyme engineering / Polyester Hydrolase Leipzig 7

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