EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis for TORC2 activation.
ジャーナル・号・ページ	Mol Cell, Vol. 86, Issue 8, Page 1560-11573.e5, Year 2026
掲載日	2026年4月16日
著者	Luoming Zou / Maria G Tettamanti / Caroline Gabus / Ariane Bergmann / Robbie Loewith / Lucas Tafur /
PubMed 要旨	The target of rapamycin complex 2 (TORC2) is a central node in signaling feedback loops, serving to maintain the biophysical homeostasis of the plasma membrane (PM). How TORC2 is regulated by ...The target of rapamycin complex 2 (TORC2) is a central node in signaling feedback loops, serving to maintain the biophysical homeostasis of the plasma membrane (PM). How TORC2 is regulated by mechanical perturbation of the PM is not well understood. To address this, we determined the cryo-electron microscopy structure of endogenous yeast TORC2 at up to 2.2 Å resolution. Our model refines the position and interactions of TORC2-specific subunits, providing a structural basis for the differential assembly of Tor2 into TORC2. Furthermore, we observe the insertion of the pleckstrin-homology domain of the Avo1 subunit into the Tor2 active site, providing a regulatory mechanism mediated by phosphoinositides. Structure-guided functional experiments reveal a potential TORC2 membrane-binding surface and a positively charged pocket in the Avo3 subunit that is necessary for TORC2 activation. Collectively, our data suggest that signaling phosphoinositides activate TORC2 by membrane-induced structural rearrangements via the concerted action of conserved regulatory subunits.
リンク	Mol Cell / PubMed:41997113
手法	EM (単粒子)
解像度	2.2 - 3.83 Å
構造データ	54222 9rsq EMDB-54222, PDB-9rsq: Cryo-EM structure of the Target of Rapamycin Complex 2 (TORC2) with the Avo1 PH domain (monomer) 手法: EM (単粒子) / 解像度: 3.07 Å 54223 9rss EMDB-54223, PDB-9rss: Cryo-EM structure of the Target of Rapamycin Complex 2 (TORC2) with the Avo1 PH domain 手法: EM (単粒子) / 解像度: 3.01 Å 54224 9rst EMDB-54224, PDB-9rst: Cryo-EM structure of the Target of Rapamycin Complex 2 (TORC2) in autoinhibted conformation (monomer) 手法: EM (単粒子) / 解像度: 2.93 Å EMDB-54227: Cryo-EM structure of the Target of Rapamycin Complex 2 (TORC2) (consensus refinement) 手法: EM (単粒子) / 解像度: 2.4 Å EMDB-54228: Cryo-EM structure of the Target of Rapamycin Complex 2 (TORC2) (monomer focused) 手法: EM (単粒子) / 解像度: 2.32 Å EMDB-54229: Cryo-EM structure of the Target of Rapamycin Complex 2 (TORC2) (Avo3 focused) 手法: EM (単粒子) / 解像度: 2.2 Å EMDB-54230: Cryo-EM structure of the Target of Rapamycin Complex 2 (TORC2) (Bit61 focused) 手法: EM (単粒子) / 解像度: 2.7 Å EMDB-54231: Cryo-EM structure of the Target of Rapamycin Complex 2 (TORC2) with Avo1 PH (Lst8 monomer focused) 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-54232: Cryo-EM structure of the Target of Rapamycin Complex 2 (TORC2) in autoinhibited conformation (monomer Lst8 focused) 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-54233: Cryo-EM structure of the Target of Rapamycin Complex 2 (TORC2) (Lst8-Avo1 CRIM focused) 手法: EM (単粒子) / 解像度: 3.83 Å EMDB-54234: Cryo-EM structure of the Target of Rapamycin Complex 2 (TORC2) with the Avo1 PH domain (composite map) 手法: EM (単粒子) / 解像度: 3.07 Å EMDB-54235: Cryo-EM structure of the Target of Rapamycin Complex 2 (TORC2) in autoinhibited conformation (composite map) 手法: EM (単粒子) / 解像度: 2.93 Å
化合物	ChemComp-IHP: INOSITOL HEXAKISPHOSPHATE
由来	saccharomyces cerevisiae (パン酵母)
キーワード	SIGNALING PROTEIN / Cell growth / metabolism