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Structure paper

TitleStructural insights into RNase H catalytic mechanism from room-temperature X-ray and neutron crystallography of apo- and RNA/DNA hybrid-bound enzyme.
Journal, issue, pagesCurr Res Struct Biol, Vol. 11, Page 100188-100188, Year 2026
Publish dateOct 3, 2025 (structure data deposition date)
AuthorsGerlits, O. / Collins, A. / Kovalevsky, A.
External linksCurr Res Struct Biol / PubMed:41853683
MethodsX-ray diffraction / neutron diffraction
Resolution1.8 - 2.48 Å
Structure data

PDB-9yjl:
Joint X-ray/neutron structure of wild-type Bacillus halodurans RNase H1 in the apo-form
Method: X-RAY DIFFRACTION / NEUTRON DIFFRACTION / Resolution: 2.48 Å

PDB-9yjm:
Joint X-ray/neutron structure of D132N Bacillus halodurans RNase H1 in the apo-form
Method: X-RAY DIFFRACTION / NEUTRON DIFFRACTION / Resolution: 2.4 Å

PDB-9yk1:
Room-temperature X-ray structure of D132N Bacillus halodurans RNase H1 in complex with RNA/DNA duplex
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-9yk3:
Room-temperature X-ray structure of D132N Bacillus halodurans RNase H1 in complex with complementary RNA/DNA duplex
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-9yk5:
100K X-ray structure of mixed metal D132N Bacillus halodurans RNase H1 complex with RNA/DNA duplex
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-SO4:
SULFATE ION

ChemComp-DOD:
DEUTERATED WATER

ChemComp-MG:
Unknown entry

ChemComp-PO3:
PHOSPHITE ION

ChemComp-HOH:
WATER

ChemComp-MN:
Unknown entry

Source
  • halalkalibacterium halodurans (bacteria)
KeywordsHYDROLASE / rna hydrolase / nucleic acid binding / HYDROLASE/RNA/DNA / RNA/DNA hybrid / HYDROLASE-RNA-DNA complex / mixed metal complex

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