EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis of lipid-linked galactan export by the mycobacterial ABC transporter Wzm-Wzt.
ジャーナル・号・ページ	Nat Commun, Year 2026
掲載日	2025年3月12日 (構造データの登録日)
著者	Alisa A Garaeva / Viktória Fabianová / Karin Savková / Stanislav Huszár / Xiaochao Xue / Todd L Lowary / Katarína Mikušová / Markus A Seeger /
PubMed 要旨	Mycobacteria, including Mycobacterium tuberculosis, possess a unique cell envelope containing arabinogalactan, a heteropolysaccharide critical for cell wall integrity and target of several ...Mycobacteria, including Mycobacterium tuberculosis, possess a unique cell envelope containing arabinogalactan, a heteropolysaccharide critical for cell wall integrity and target of several tuberculosis drugs. The cytosolic precursor of arabinogalactan, lipid-linked galactan (LLG), is translocated across the plasma membrane by the essential ABC transporter Wzm-Wzt through a molecular mechanism that is poorly understood. Here, we present a series of cryo-EM structures of Wzm-Wzt from Mycobacterium abscessus, representing different conformations of the transport cycle. Conserved residues lining the proposed LLG translocation pathway were investigated by three orthologous functional assays, revealing that the cytosolic gate helix (GH) plays a key functional role in polysaccharide transport. Our data suggests that the hydrophobic polyprenyl-moiety is translocated first, followed by the galactan-polysaccharide, which requires Wzm-Wzt to open a continuous channel through which the sugar chain is ratcheted at the expense of ATP hydrolysis. Our results provide a rational basis for the development of drugs that inhibit mycobacterial cell wall biosynthesis.
リンク	Nat Commun / PubMed:41839883
手法	EM (単粒子)
解像度	3.3 - 4.04 Å
構造データ	PDB-9qfx: Nucleotide-free wild type Wzm-Wzt from Mycobacterium abscessus in LMNG 手法: ELECTRON MICROSCOPY / 解像度: 3.45 Å PDB-9qgu: ADP-bound wild type Wzm-Wzt from Mycobacterium abscessus in LMNG 手法: ELECTRON MICROSCOPY / 解像度: 3.69 Å PDB-9qh1: ATP-bound wild type Wzm-Wzt from Mycobacterium abscessus in LMNG 手法: ELECTRON MICROSCOPY / 解像度: 3.44 Å PDB-9qhj: ATP-bound E178Q Wzm-Wzt from Mycobacterium abscessus in LMNG 手法: ELECTRON MICROSCOPY / 解像度: 3.4 Å PDB-9qhk: Nucleotide-free wild type Wzm-Wzt from Mycobacterium abscessus in nanodiscs 手法: ELECTRON MICROSCOPY / 解像度: 3.57 Å PDB-9qhv: ADP-bound wild type Wzm-Wzt from Mycobacterium abscessus in nanodiscs 手法: ELECTRON MICROSCOPY / 解像度: 4.04 Å PDB-9qhw: ATP-bound wild type Wzm-Wzt from Mycobacterium abscessus in nanodiscs 手法: ELECTRON MICROSCOPY / 解像度: 3.3 Å PDB-9qhx: ATP-bound E178Q Wzm-Wzt from Mycobacterium abscessus in nanodiscs 手法: ELECTRON MICROSCOPY / 解像度: 3.47 Å
化合物	ChemComp-AV0: Lauryl Maltose Neopentyl Glycol / ラウリルマルト-スネオペンチルグリコ-ル ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-PTY: PHOSPHATIDYLETHANOLAMINE / リン脂質YM 化合物画像なし ChemComp-UNL*: Unknown ligand
由来	mycobacteroides abscessus (バクテリア)
キーワード	MEMBRANE PROTEIN / ABC transporter Type V ABC transporter Arabinogalactan precursor Lipid-linked galactan exporter