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TitleBacterial immune activation via supramolecular assembly with phage triggers.
Journal, issue, pagesNature, Vol. 651, Issue 8107, Page 1051-1059, Year 2026
Publish dateFeb 4, 2026
AuthorsTong Zhang / Yifei Lyu / Christina R Beck / Naseer Iqbal / Renee Barbosa / Alireza Ghanbarpour / Michael T Laub /
PubMed AbstractBacteria use diverse mechanisms to protect themselves against phages. Many antiphage systems form large oligomeric complexes, but how oligomerization is regulated during phage infection remains ...Bacteria use diverse mechanisms to protect themselves against phages. Many antiphage systems form large oligomeric complexes, but how oligomerization is regulated during phage infection remains mostly unknown. Here we demonstrate that the bacterial immunity protein ring-activated zinc-finger RNase (RAZR) assembles into an active, 24-meric ring around the circumference of large ring structures formed by two unrelated phage proteins: a putative recombinase and a portal protein. Each multi-layered, megadalton-scale complex enables RAZR to cleave RNA nonspecifically to inhibit translation and restrict phage propagation. The recognition of unrelated phage proteins that form rings with similar diameters indicates that these proteins not only bind to RAZR but also enforce a geometry crucial to activation. The lack of large ring structures in the host probably prevents auto-immunity and RAZR activation before infection. The infection-triggered oligomerization of RAZR mirrors pathogen-induced oligomerization in eukaryotic innate immune complexes, underscoring a common principle of immunity across biology.
External linksNature / PubMed:41639456 / PubMed Central
MethodsEM (single particle)
Resolution3.4 - 3.5 Å
Structure data

EMDB-72691: Cryo-EM Map of the Copurified Ring-Activated Zinc-Finger RNase (RAZR, H154A) in Complex with Phage Protein Gp77
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-72692: Focused-Refinement Cryo-EM Map of the RAZR-ZFD Domain within the In Vitro Reconstituted RAZR.Gp77 Complex
Method: EM (single particle) / Resolution: 3.5 Å

72693

9y9c

EMDB-72693, PDB-9y9c:
Cryo-EM map of the in vitro reconstituted RAZR:GP77 complex with AlphaFold-predicted models fitted into the density.
Method: EM (single particle) / Resolution: 3.5 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • BL21 DE3 (bacteria)
  • Escherichia coli BL21(DE3) (bacteria)
  • escherichia phage secphi27 (virus)
  • escherichia coli (E. coli)
KeywordsRNA BINDING PROTEIN / Phage-bacterial defense complex / Abortive infection Ring-Activated Zinc-Finger RNase (RAZR)

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