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-Structure paper
| タイトル | In situ molecular organization and heterogeneity of the Legionella Dot/Icm T4SS. |
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| ジャーナル・号・ページ | FEBS Lett, Year 2026 |
| 掲載日 | 2026年1月9日 |
著者 | Przemysław Dutka / Yuxi Liu / Stefano Maggi / Debnath Ghosal / Jue Wang / Prashant P Damke / Stephen D Carter / Wei Zhao / Sukhithasri Vijayrajratnam / Joseph P Vogel / Carrie L Shaffer / Grant J Jensen / ![]() |
| PubMed 要旨 | The Dot/Icm type IV secretion system (T4SS) is essential for Legionella pneumophila infection, but its in situ architecture and mechanism remain incompletely understood. Using cryo-electron ...The Dot/Icm type IV secretion system (T4SS) is essential for Legionella pneumophila infection, but its in situ architecture and mechanism remain incompletely understood. Using cryo-electron tomography, we performed subtomogram averaging and 3D classification to resolve structural heterogeneity within the complex. We identified multiple assembly states of the inner membrane complex, including a fully assembled form with a hexamer-of-dimers DotO ATPase and symmetry mismatches between subcomplexes. A composite in situ model revealed a central channel above the inner membrane, likely used for substrate secretion. Imaging of infected macrophages showed T4SSs tethered to host vacuoles and extracellular vesicle release, suggesting additional effector delivery routes. These findings provide insight into Dot/Icm T4SS structure and infection-related dynamics. |
リンク | FEBS Lett / PubMed:41510630 |
| 手法 | EM (サブトモグラム平均) |
| 解像度 | 21.0 - 30.0 Å |
| 構造データ | ![]() EMDB-71739: Legionella Dot/Icm T4SS ![]() EMDB-72186: Focused refinement map of the periplasmic part of the Legionella pneumophila T4SS. ![]() EMDB-72187: Focused refinement map of the cytoplasmic region of the Legionella pneumophila T4SS. |
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