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TitleA Robust Bioprocess for the Global Incorporation of Noncanonical Amino Acids in Auxotrophic Hosts Produces Labeled Proteins at the Gram Scale.
Journal, issue, pagesChembiochem, Vol. 27, Page e202500669-e202500669, Year 2026
Publish dateOct 9, 2024 (structure data deposition date)
AuthorsFladischer, P. / Anderhuber, N. / Murgu, O. / Hromic-Jahjefendic, A. / Pavkov-Keller, T. / Gruber, K. / Mairhofer, J. / Striedner, G. / Wiltschi, B.
External linksChembiochem / PubMed:41376136
MethodsX-ray diffraction
Resolution2.79 Å
Structure data

PDB-9h0z:
Crystal structure of TTL[Nle], thermophilic lipase TTL from Thermoanaerobacter thermohydrosulfuricus containing non-canonical amino acid Nle at the position of Met
Method: X-RAY DIFFRACTION / Resolution: 2.79 Å

Chemicals

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

Source
  • thermoanaerobacter thermohydrosulfuricus (bacteria)
KeywordsLIPID BINDING PROTEIN / lipase / thermophilic / non-canonical amino acid / Nle / ncAAs / norleucine

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