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Structure paper

TitleSpitrobot-2 advances time-resolved cryo-trapping crystallography to under 25 ms.
Journal, issue, pagesCommun Chem, Vol. 8, Page 363-363, Year 2025
Publish dateMay 7, 2025 (structure data deposition date)
AuthorsSpiliopoulou, M. / Hatton, C.E. / Kollewe, M. / Leimkohl, J.P. / Schikora, H. / Tellkamp, F. / Mehrabi, P. / Schulz, E.C.
External linksCommun Chem / PubMed:41266557
MethodsX-ray diffraction
Resolution1.3 - 2.1 Å
Structure data

PDB-9r45:
Spitrobot-2 advances time-resolvedcryo-trapping crystallography to under 25 ms: Xylose isomerase (apo state)
Method: X-RAY DIFFRACTION / Resolution: 1.79 Å

PDB-9r46:
Spitrobot-2 advances time-resolvedcryo-trapping crystallography to under 25 ms: Xylose isomerase bound with glucose (25 ms soaking)
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-9r47:
Spitrobot-2 advances time-resolvedcryo-trapping crystallography to under 25 ms: Xylose isomerase bound with glucose (50 ms soaking)
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-9r48:
Spitrobot-2 advances time-resolvedcryo-trapping crystallography to under 25 ms: Human insulin, pH 9.0 (apo state)
Method: X-RAY DIFFRACTION / Resolution: 1.65 Å

PDB-9r49:
Spitrobot-2 advances time-resolvedcryo-trapping crystallography to under 25 ms: Human insulin, pH 4.5 (25 ms soaking)
Method: X-RAY DIFFRACTION / Resolution: 1.641 Å

PDB-9r4a:
Spitrobot-2 advances time-resolvedcryo-trapping crystallography to under 25 ms: Human insulin, pH 4.5 (50 ms soaking)
Method: X-RAY DIFFRACTION / Resolution: 1.45 Å

PDB-9r4b:
Spitrobot-2 advances time-resolvedcryo-trapping crystallography to under 25 ms: Human insulin, pH 4.5 (250 ms soaking)
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-9r4c:
Spitrobot-2 advances time-resolvedcryo-trapping crystallography to under 25 ms: Human insulin, pH 4.5 (500 ms soaking)
Method: X-RAY DIFFRACTION / Resolution: 1.41 Å

PDB-9r4e:
Spitrobot-2 advances time-resolvedcryo-trapping crystallography to under 25 ms: Human insulin, pH 4.5 (5 s soaking)
Method: X-RAY DIFFRACTION / Resolution: 1.3 Å

PDB-9r4f:
Spitrobot-2 advances time-resolvedcryo-trapping crystallography to under 25 ms: T4 Lysozyme, mutant L99A (apo state)
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-9r4g:
Spitrobot-2 advances time-resolvedcryo-trapping crystallography to under 25 ms: T4 Lysozyme, mutant L99A bound with indole (1 s soaking)
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-9r4h:
Spitrobot-2 advances time-resolvedcryo-trapping crystallography to under 25 ms: T4 Lysozyme, mutant L99A bound with indole (10 s soaking)
Method: X-RAY DIFFRACTION / Resolution: 1.49 Å

Chemicals

ChemComp-MN:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-MG:
Unknown entry

ChemComp-BGC:
beta-D-glucopyranose

ChemComp-SO4:
SULFATE ION

ChemComp-IND:
INDOLE

ChemComp-NA:
Unknown entry

Source
  • streptomyces rubiginosus (bacteria)
  • homo sapiens (human)
  • escherichia phage t4 (virus)
KeywordsISOMERASE / Xylose isomerase / Spitrobot-2 / glucose / HORMONE / Human insulin / pH-jump / pH 4.5 / HYDROLASE / T4 lysozyme / L99A / indole

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