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-Structure paper
| タイトル | Structural basis of bacteriophage Ur-lambda infection initiation. |
|---|---|
| ジャーナル・号・ページ | Sci Adv, Vol. 11, Issue 46, Page eadw7914, Year 2025 |
| 掲載日 | 2025年11月14日 |
著者 | Huaxin Yu / Chunyan Wang / Jian Yue / Wangbiao Guo / Ian J Molineux / Jun Liu / ![]() |
| PubMed 要旨 | Bacteriophages must recognize host receptors and penetrate the host cell envelope to initiate infection. How the classic phage λ initiates infection is not yet understood. Here, we combine cryo- ...Bacteriophages must recognize host receptors and penetrate the host cell envelope to initiate infection. How the classic phage λ initiates infection is not yet understood. Here, we combine cryo-electron microscopy and tomography to visualize infection initiation by Ur-λ, the original λ isolate that uses side fibers to adsorb rapidly to . We determine the structure of Ur-λ, resolving the full-length central and side fibers, thus providing a structural basis for host recognition. We show that Ur-λ contains six copies of its tape measure protein. We capture intermediates of the tail tip complex during infection initiation, revealing how extensive conformational changes enable adsorption, and visualize the trans-envelope channel required for genome ejection. |
リンク | Sci Adv / PubMed:41237242 / PubMed Central |
| 手法 | EM (単粒子) |
| 解像度 | 3.31 - 3.37 Å |
| 構造データ | EMDB-47685, PDB-9e7m: EMDB-48256, PDB-9mgh: |
| 化合物 | ![]() ChemComp-SF4: |
| 由来 |
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キーワード | VIRAL PROTEIN / bacteriophage / tail tip complex |
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escherichia phage lambda (λファージ)
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