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Structure paper

TitleProteasome-associated autoinflammatory syndromes: The impact of mutations in proteasome subunits on particle assembly, structure, and activity.
Journal, issue, pagesStructure, Vol. 33, Page 2084-, Year 2025
Publish dateFeb 28, 2025 (structure data deposition date)
AuthorsHuber, E.M. / Heinemeyer, W. / Groll, M.
External linksStructure / PubMed:41033310
MethodsX-ray diffraction
Resolution2.6 - 2.9 Å
Structure data

PDB-9qaf:
Yeast 20S proteasome mutant: beta5_T3M
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

PDB-9qai:
Yeast 20S proteasome mutant: beta5_T3M in complex with MG132
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

PDB-9qb1:
Yeast 20S proteasome mutant: beta5_T3M in complex with Bortezomib
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

PDB-9qb4:
Yeast 20S proteasome mutant: beta5_T3M in complex with Carfilzomib
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

PDB-9qbe:
Yeast 20S proteasome mutant: beta5_T3M in complex with ONX0914
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

PDB-9qbi:
Yeast 20S proteasome mutant: beta5_G128V (b5-propeptide in trans)
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

PDB-9qbo:
Yeast 20S proteasome mutant: beta5_G128V (b5-propeptide in trans) in complex with MG132
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

PDB-9qbw:
Yeast 20S proteasome mutant: beta5_G128V (b5-propeptide in trans) in complex with Carfilzomib
Method: X-RAY DIFFRACTION / Resolution: 2.9 Å

PDB-9qby:
Yeast 20S proteasome mutant: beta5_G128V (b5-propeptide in trans) in complex with ONX0914
Method: X-RAY DIFFRACTION / Resolution: 2.9 Å

PDB-9qc9:
Yeast 20S proteasome double mutant: beta5_F(-46)S / beta5_G128V
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

PDB-9qcf:
Yeast 20S proteasome mutant: beta1_G128V
Method: X-RAY DIFFRACTION / Resolution: 2.9 Å

PDB-9qck:
Yeast 20S proteasome mutant: beta1_G128V (b1-propeptide deleted)
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

PDB-9qd2:
Yeast 20S proteasome mutant: beta1_G128V (b1-propeptide deleted) in complex with MG132
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

PDB-9qdf:
Yeast 20S proteasome mutant: beta1_G128V (b1-propeptide deleted) in complex with Bortezomib
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

PDB-9qds:
Yeast 20S proteasome mutant: beta1_G128V (b1-propeptide deleted) in complex with Carfilzomib
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-MES:
2-(N-MORPHOLINO)-ETHANESULFONIC ACID / pH buffer*YM

ChemComp-SO4:
SULFATE ION

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM

Source
  • saccharomyces cerevisiae (brewer's yeast)
  • synthetic construct (others)
KeywordsHYDROLASE / Proteasome / Proteasome Associated Autoinflammatory Syndromes / Mutation / Assembly / Maturation

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