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TitleCryo-EM structure and dynamic basis of phosphate uptake by PHT1 in rice.
Journal, issue, pagesDev Cell, Year 2025
Publish dateSep 25, 2025
AuthorsZhangmeng Du / Zeyuan Guan / Hai Liu / Jie Zhang / Haitao He / Zhiwen Zheng / Wenhui Zhang / Lihuan Jiang / Jiaqi Zuo / Yan Liu / Beijing Wan / Haifu Tu / Faming Dong / Xuelei Lai / Lizhong Xiong / Ping Yin / Shaowu Xue / Yanke Chen / Zhu Liu /
PubMed AbstractPhosphorus is an essential macronutrient for plants, primarily absorbed from the soil as inorganic phosphate (Pi) through root-located Pi transporters. Despite decades of research into these ...Phosphorus is an essential macronutrient for plants, primarily absorbed from the soil as inorganic phosphate (Pi) through root-located Pi transporters. Despite decades of research into these transporters as targets for developing Pi-efficient crops, their mechanisms for Pi import remain poorly understood. Here, we present the cryo-electron microscopy (cryo-EM) structures of the rice Pi importer OsPHT1;11 in both Pi-bound and unbound forms, characterize its conformational dynamics, and demonstrate how these dynamics contribute to its transport function. Pi is recognized through conserved residues found in plants, with its translocation facilitated by a typical alternating-access mechanism. Single-molecule fluorescence resonance energy transfer (smFRET) analyses show that this transporter undergoes dynamic conformational fluctuations, which are differentially linked to its Pi transport capability, with a predominance of extracellular open conformations favoring Pi transport, while more populated intracellular open conformations hinder it. These findings highlight key conformational determinants of transport activity and provide mechanistic insights into Pi uptake in plants.
External linksDev Cell / PubMed:41005295
MethodsEM (single particle)
Resolution3.3 - 3.4 Å
Structure data

62441

9kmq

EMDB-62441, PDB-9kmq:
CryoEM structure of osPHT1-11 at pH 8.0
Method: EM (single particle) / Resolution: 3.3 Å

62480

9kou

EMDB-62480, PDB-9kou:
CryoEM structure of osPHT1-11 at pH 5.0
Method: EM (single particle) / Resolution: 3.4 Å

Chemicals

ChemComp-PO4:
PHOSPHATE ION

Source
  • oryza sativa subsp. japonica (Japanese rice)
  • oryza sativa japonica group (Japanese rice)
KeywordsTRANSPORT PROTEIN / phosphorus / rice / uptake

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