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Structure paper

TitleResidues in the little finger domain of the Y-family Dpo4 DNA polymerase communicate to restrict synthesis past 8-oxoguanine lesions.
Journal, issue, pagesNucleic Acids Res., Vol. 53, Year 2025
Publish dateFeb 28, 2025 (structure data deposition date)
AuthorsDisha, S.S. / Punchipatabendi, T.I. / Kaszubowski, J.D. / Liang, B. / Pata, J.D. / Trakselis, M.A.
External linksNucleic Acids Res. / PubMed:41002026
MethodsX-ray diffraction
Resolution2.5 - 2.9 Å
Structure data

PDB-9nkc:
Dpo4 DNA polymerase (Wild Type) in complex with DNA containing an 8oxoG template lesion
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-9nkd:
Dpo4 DNA polymerase (R332A) in complex with DNA containing an 8oxoG template lesion
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-9nke:
Dpo4 DNA polymerase (R336A) in complex with DNA containing an 8oxoG template lesion
Method: X-RAY DIFFRACTION / Resolution: 2.9 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-DTP:
2'-DEOXYADENOSINE 5'-TRIPHOSPHATE

ChemComp-HOH:
WATER

ChemComp-PO4:
PHOSPHATE ION

Source
  • saccharolobus solfataricus p2 (archaea)
  • synthetic construct (others)
  • saccharolobus solfataricus (archaea)
KeywordsTRANSFERASE/DNA / DNA polymerase Translesion synthesis 8oxoG damage / TRANSFERASE / TRANSFERASE-DNA complex

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