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Structure paper

TitlePropagation of Photoinduced Electric Field Changes Through Phytochrome and their Impact on Conformational Transitions.
Journal, issue, pagesChemphyschem, Vol. 26, Page e202500595-e202500595, Year 2025
Publish dateJun 19, 2025 (structure data deposition date)
AuthorsLa Greca, M. / Nguyen, A.D. / Kraskov, A. / Michael, N. / Sauthof, L. / Ebrahim, M. / Katz, S. / von Sass, J. / Hoang, O.T. / Budisa, N. ...La Greca, M. / Nguyen, A.D. / Kraskov, A. / Michael, N. / Sauthof, L. / Ebrahim, M. / Katz, S. / von Sass, J. / Hoang, O.T. / Budisa, N. / Scheerer, P. / Schlesinger, R. / Mroginski, M.A. / Hildebrandt, P.
External linksChemphyschem / PubMed:40976591
MethodsX-ray diffraction
Resolution1.79 Å
Structure data

PDB-9rn8:
Crystal structure of the photosensory core module (PCM) of a cyano-phenylalanine mutant oCNF205 of the bathy phytochrome Agp2 from Agrobacterium fabrum in the Pfr state.
Method: X-RAY DIFFRACTION / Resolution: 1.79 Å

Chemicals

ChemComp-EL5:
3-[(2Z)-2-({3-(2-carboxyethyl)-5-[(E)-(4-ethenyl-3-methyl-5-oxo-1,5-dihydro-2H-pyrrol-2-ylidene)methyl]-4-methyl-1H-pyrrol-2-yl}methylidene)-5-{(Z)-[(3E,4S)-3-ethylidene-4-methyl-5-oxopyrrolidin-2-ylidene]methyl}-4-methyl-2H-pyrrol-3-yl]propanoic acid

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-MES:
2-(N-MORPHOLINO)-ETHANESULFONIC ACID / pH buffer*YM

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

Source
  • agrobacterium fabrum str. c58 (bacteria)
KeywordsSIGNALING PROTEIN / fluorescent protein / phytochrome / bacteriophytochrome / Pfr-state / ortho-cyano-phenylalanine / Stark labels

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