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Structure paper

TitleProbing the extent of importin-alpha targeting of the TAF8 NLS by eliminating its cationic net-charge.
Journal, issue, pagesProtein Sci., Vol. 34, Page e70272-e70272, Year 2025
Publish dateJul 10, 2024 (structure data deposition date)
AuthorsAbdoli, A. / Yang, Z. / Odeh-Ahmed, A. / Bednova, O. / Lemieux, B. / Dawe, L. / Ravel-Chapuis, A. / Lavigne, P. / Zeytuni, N. / Leyton, J.V.
External linksProtein Sci. / PubMed:40852899
MethodsX-ray diffraction
Resolution2.11 - 2.42 Å
Structure data

PDB-9cl8:
Importin alpha isoform 2 with synthetic zero net-charge nuclear localization signal
Method: X-RAY DIFFRACTION / Resolution: 2.11 Å

PDB-9cle:
Importin alpha isoform 2 with synthetic zero net-charge nuclear localization signal
Method: X-RAY DIFFRACTION / Resolution: 2.34 Å

PDB-9clf:
Importin alpha isoform 2 with synthetic zero net-charge nuclear localization signal
Method: X-RAY DIFFRACTION / Resolution: 2.36 Å

PDB-9clg:
Importin alpha isoform 2 with synthetic zero net-charge nuclear localization signal
Method: X-RAY DIFFRACTION / Resolution: 2.42 Å

Chemicals

ChemComp-GOL:
GLYCEROL

ChemComp-DTT:
2,3-DIHYDROXY-1,4-DITHIOBUTANE

ChemComp-HOH:
WATER

Source
  • mus musculus (house mouse)
  • synthetic construct (others)
KeywordsPROTEIN TRANSPORT / Nuclear transport / nuclear localization signal binding / ARM repeats

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