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Structure paper

TitleD-cysteine impairs tumour growth by inhibiting cysteine desulfurase NFS1.
Journal, issue, pagesNat Metab, Year 2025
Publish dateAug 18, 2023 (structure data deposition date)
AuthorsZangari, J. / Stehling, O. / Freibert, S.A. / Bhattacharya, K. / Rouaud, F. / Serre-Beinier, V. / Maundrell, K. / Montessuit, S. / Ferre, S.M. / Vartholomaiou, E. ...Zangari, J. / Stehling, O. / Freibert, S.A. / Bhattacharya, K. / Rouaud, F. / Serre-Beinier, V. / Maundrell, K. / Montessuit, S. / Ferre, S.M. / Vartholomaiou, E. / Schulz, V. / Zuhra, K. / Gonzalez-Ruiz, V. / Hanschke, S. / Tsukamoto, T. / Cerezo, M. / Szabo, C. / Rudaz, S. / Boniecki, M.T. / Cygler, M. / Lill, R. / Martinou, J.C.
External linksNat Metab / PubMed:40797101
MethodsX-ray diffraction
Resolution2 Å
Structure data

PDB-8tvt:
Structure of human Cysteine desulfurase Nfs1 with L-propargylglycine bound to active site PLP in complex with ISD11, Acp1 and ISCU2
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-PLP:
PYRIDOXAL-5'-PHOSPHATE

ChemComp-LPH:
L-Propargylglycine

ChemComp-GOL:
GLYCEROL

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

ChemComp-P15:
2,5,8,11,14,17-HEXAOXANONADECAN-19-OL

ChemComp-DTT:
2,3-DIHYDROXY-1,4-DITHIOBUTANE

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-EDT:
{[-(BIS-CARBOXYMETHYL-AMINO)-ETHYL]-CARBOXYMETHYL-AMINO}-ACETIC ACID

ChemComp-8Q1:
S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate

ChemComp-MES:
2-(N-MORPHOLINO)-ETHANESULFONIC ACID / pH buffer*YM

ChemComp-1PE:
PENTAETHYLENE GLYCOL / precipitant*YM

ChemComp-HOH:
WATER

Source
  • homo sapiens (human)
  • escherichia coli (E. coli)
KeywordsTRANSFERASE / Nfs1 / PG-PLP bound

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