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Structure paper

TitleFunctional and structural insights into a thermostable (S)-selective amine transaminase and its improved substrate scope by protein engineering.
Journal, issue, pagesAppl. Microbiol. Biotechnol., Vol. 109, Page 180-180, Year 2025
Publish dateAug 15, 2024 (structure data deposition date)
AuthorsPatti, S. / De Rose, S.A. / Isupov, M.N. / Magrini Alunno, I. / Riva, S. / Ferrandi, E.E. / Littlechild, J.A. / Monti, D.
External linksAppl. Microbiol. Biotechnol. / PubMed:40796981
MethodsX-ray diffraction
Resolution1.24 - 1.49 Å
Structure data

PDB-9gh9:
A novel aminotransferase from Streptomyces sp.
Method: X-RAY DIFFRACTION / Resolution: 1.49 Å

PDB-9gnf:
A novel aminotransferase from Streptomyces sp. Gabaculine complex
Method: X-RAY DIFFRACTION / Resolution: 1.31 Å

PDB-9qc2:
BV333 aminotransferase from Streptomyces sp. mutant W89A
Method: X-RAY DIFFRACTION / Resolution: 1.24 Å

PDB-9qgh:
BV333 aminotransferase from Streptomyces sp. mutant F61C
Method: X-RAY DIFFRACTION / Resolution: 1.31 Å

Chemicals

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-PLP:
PYRIDOXAL-5'-PHOSPHATE

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

ChemComp-PXG:
3-[O-PHOSPHONOPYRIDOXYL]--AMINO-BENZOIC ACID

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-CA:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-GOL:
GLYCEROL

ChemComp-1PG:
2-(2-{2-[2-(2-METHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHOXY)-ETHANOL

ChemComp-MG:
Unknown entry

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

Source
  • streptomyces sp. bv333 (bacteria)
KeywordsTRANSFERASE / Aminotransferase / SBV333

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