EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Measuring the selective packaging of RNA molecules by viral coat proteins in cells.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 122, Issue 33, Page e2505190122, Year 2025
掲載日	2025年8月19日
著者	Amineh Rastandeh / Nino Makasarashvili / Herman K Dhaliwal / Sherry Baker / Sundharraman Subramanian / Daniel A Villarreal / Elmer I Gamez / Kristin N Parent / Rees F Garmann /
PubMed 要旨	Some RNA viruses package their genomes with extraordinary selectivity, assembling protein capsids around their own viral RNA while excluding nearly all host RNA. How the assembling proteins ...Some RNA viruses package their genomes with extraordinary selectivity, assembling protein capsids around their own viral RNA while excluding nearly all host RNA. How the assembling proteins distinguish viral RNA from host RNA is not fully understood, but RNA structure is thought to play a key role. To test this idea, we perform in-cellulo packaging experiments using bacteriophage MS2 coat proteins and a variety of RNA molecules in . In each experiment, plasmid-derived RNA molecules with a specified sequence compete against the cellular transcriptome for packaging by plasmid-derived coat proteins. Following this competition, we quantify the total amount and relative composition of the packaged RNA using electron microscopy, interferometric scattering microscopy, and high-throughput sequencing. By systematically varying the input RNA sequence and measuring changes in packaging outcomes, we are able to directly test competing models of selective packaging. Our results rule out a longstanding model in which selective packaging requires the well-known translational repressor (TR) stem-loop, and instead support more recent models in which selectivity emerges from the collective interactions of multiple coat proteins and multiple stem-loops distributed across the RNA molecule. These findings establish a framework for studying and understanding selective packaging in a range of natural viruses and virus-like particles, and lay the groundwork for engineering synthetic systems that package specific RNA cargoes.
リンク	Proc Natl Acad Sci U S A / PubMed:40789029 / PubMed Central
手法	EM (単粒子)
解像度	2.2 - 3.5 Å
構造データ	48864 9n40 EMDB-48864, PDB-9n40: MS2-pMS2 Icosahedral Reconstruction 手法: EM (単粒子) / 解像度: 2.4 Å 48865 9n41 EMDB-48865, PDB-9n41: MS2-pcoat Icosahedral Reconstruction 手法: EM (単粒子) / 解像度: 2.2 Å EMDB-48866: MS2-pMS2 D5 reconstruction 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-48867: MS2-pcoat D5 reconstruction 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-48868: MS2-pcoat C1 reconstruction 手法: EM (単粒子) / 解像度: 3.5 Å
由来	escherichia phage ms2 (ファージ)
キーワード	VIRUS LIKE PARTICLE / MS2 / VIRUS