EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis for promoter recognition and transcription factor binding and release in human mitochondria.
ジャーナル・号・ページ	Mol Cell, Vol. 85, Issue 16, Page 3123-33136.e7, Year 2025
掲載日	2025年8月21日
著者	Karl Herbine / Ashok R Nayak / Angelica Zamudio-Ochoa / Dmitry Temiakov /
PubMed 要旨	Transcription in human mitochondria is driven by a core apparatus consisting of a Pol A family RNA polymerase (mtRNAP), the initiation factors TFAM and TFB2M, and the elongation factor TEFM. While ...Transcription in human mitochondria is driven by a core apparatus consisting of a Pol A family RNA polymerase (mtRNAP), the initiation factors TFAM and TFB2M, and the elongation factor TEFM. While earlier structures of initiation and elongation complexes provided valuable snapshots, they represent isolated stages of a highly dynamic and multistep process. Critical aspects of mitochondrial transcription-such as DNA recognition and melting, promoter escape, and the release of initiation factors-remain poorly understood. Here, we present a series of cryoelectron microscopy (cryo-EM) structures that capture the transcription complex as it transitions from the initial open promoter complex to the processive elongation complex through intermediate stages. Our data reveal new, previously unidentified determinants of promoter specificity: the sequential disengagement of mtRNAP from TFAM and the promoter, the release of TFB2M, and the recruitment of TEFM. Together, these findings provide a detailed molecular mechanism underlying transcription in human mitochondria.
リンク	Mol Cell / PubMed:40712587 / PubMed Central
手法	EM (単粒子)
解像度	2.65 - 3.77 Å
構造データ	48412 9mn4 EMDB-48412, PDB-9mn4: Structure of the human mitochondrial initially transcribing complex, IC3 手法: EM (単粒子) / 解像度: 3.05 Å 48413 9mn5 EMDB-48413, PDB-9mn5: Structure of the human mitochondrial open transcription initiation complex, IC0 手法: EM (単粒子) / 解像度: 3.04 Å 48414 9mn6 EMDB-48414, PDB-9mn6: Structure of the human mitochondrial late-stage transcription initiation complex, IC8 手法: EM (単粒子) / 解像度: 2.71 Å 48415 9mn7 EMDB-48415, PDB-9mn7: Structure of the human mitochondrial late-stage transcription initiation complex, IC8 手法: EM (単粒子) / 解像度: 2.65 Å 48416 9mn8 EMDB-48416, PDB-9mn8: Structure of the human mitochondrial transcription initiation transitional complex, TC8 手法: EM (単粒子) / 解像度: 2.69 Å 48417 9mn9 EMDB-48417, PDB-9mn9: Structure of the human mitochondrial promoter-initiated transcription elongation complex, P-EC13 手法: EM (単粒子) / 解像度: 2.74 Å 48418 9mna EMDB-48418, PDB-9mna: Structure of the human mitochondrial promoter-initiated transcription elongation complex with TEFM, pEC9-TEFM 手法: EM (単粒子) / 解像度: 3.77 Å
化合物	ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry ChemComp-CH1*: 3'-DEOXY-CYTIDINE-5'-TRIPHOSPHATE
由来	homo sapiens (ヒト) synthetic construct (人工物)
キーワード	TRANSCRIPTION / Mitochondrial RNA Polymerase / Transcription Initiation complex / POLRMT / TRANSCRIPTION-DNA-RNA complex