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TitleAn alternate receptor for adeno-associated viruses.
Journal, issue, pagesCell, Vol. 188, Issue 18, Page 4924-4935.e23, Year 2025
Publish dateSep 4, 2025
AuthorsBijay P Dhungel / Hua Xu / Rajini Nagarajah / Joseph Vitale / Alex C H Wong / Divya Gokal / Yue Feng / Mehdi Sharifi Tabar / Cynthia Metierre / Chirag Parsania / Xiaohui Song / Guopeng Wang / Xiao-Dong Su / Charles G Bailey / John E J Rasko /
PubMed AbstractSystemic gene therapy using adeno-associated virus (AAV) vectors is approved for the treatment of several genetic disorders, but challenges and toxicities associated with high vector doses remain. We ...Systemic gene therapy using adeno-associated virus (AAV) vectors is approved for the treatment of several genetic disorders, but challenges and toxicities associated with high vector doses remain. We report an alternate receptor for AAV (AAVR2, carboxypeptidase D [CPD]), which is distinct from the multi-serotype AAV receptor (AAVR). AAVR2 enables the transduction of clade E AAVs, including AAV8, and determines an exclusive AAVR-independent transduction pathway for AAV11 and AAV12. We characterized direct binding between the AAV8 capsid and AAVR2 by cryo-electron microscopy (cryo-EM) and identified contact residues. We observed that AAV8 directly binds to the carboxypeptidase-like domain 1 of AAVR2 via its variable region VIII and demonstrated that AAV capsids that lack AAVR2 binding can be bioengineered to engage with AAVR2. Finally, we overexpressed a minimal functional AAVR2 to enhance AAV transduction in vivo. Our study provides insights into AAV biology and clinically deployable solutions to reduce dose-related toxicities associated with AAV vectors.
External linksCell / PubMed:40664211
MethodsEM (single particle)
Resolution2.32 - 3.02 Å
Structure data

61190

9j6z

EMDB-61190, PDB-9j6z:
Structure of AAV8 in complex with its receptor
Method: EM (single particle) / Resolution: 3.02 Å

61205

9j7k

EMDB-61205, PDB-9j7k:
Structure of AAV8 in the complex of AAV8 with its receptor
Method: EM (single particle) / Resolution: 2.32 Å

61206

9j7l

EMDB-61206, PDB-9j7l:
Structure of AAV8 capsid in complex with receptor
Method: EM (single particle) / Resolution: 2.89 Å

Source
  • adeno-associated virus - 8
  • homo sapiens (human)
KeywordsVIRAL PROTEIN/HYDROLASE / AAV8 / receptor / complex / VIRAL PROTEIN-HYDROLASE complex / VIRUS

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