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TitleCombined approaches to enhance the Pichia pastoris-expressed PET hydrolase.
Journal, issue, pagesInt. J. Biol. Macromol., Vol. 320, Page 145862-145862, Year 2025
Publish dateJan 20, 2025 (structure data deposition date)
AuthorsLi, X. / Huang, J.W. / Ning, Z. / Huang, S. / Zeng, C. / Zeng, Z. / Ji, R. / Peng, R. / Liu, X. / Min, J. ...Li, X. / Huang, J.W. / Ning, Z. / Huang, S. / Zeng, C. / Zeng, Z. / Ji, R. / Peng, R. / Liu, X. / Min, J. / Chen, C.C. / Guo, R.T.
External linksInt. J. Biol. Macromol. / PubMed:40653245
MethodsX-ray diffraction
Resolution1.4 - 1.71 Å
Structure data

PDB-9lms:
Crystal structure of Pichia pastoris-expressed FAST-PETase-N212A/K233C/S282C variant
Method: X-RAY DIFFRACTION / Resolution: 1.71 Å

PDB-9lmt:
Crystal structure of variant FAST-ACC-A248E
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-9lmu:
Crystal structure of variant FAST-ACC-A47E
Method: X-RAY DIFFRACTION / Resolution: 1.57 Å

PDB-9lmv:
Crystal structure of FAST-ACC-T140D/R132G/S160A mutant in complex with mono(2-hydroxyethyl) terephthalic acid
Method: X-RAY DIFFRACTION / Resolution: 1.57 Å

PDB-9lmw:
Crystal structure of variant FAST-ACC-T140E
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-9lmx:
Inactive mutant of FAST-ACC-T140E/R132G in complex with mono(2-hydroxyethyl) terephthalic acid
Method: X-RAY DIFFRACTION / Resolution: 1.4 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

ChemComp-HOH:
WATER

ChemComp-C9C:
4-(2-hydroxyethyloxycarbonyl)benzoic acid

Source
  • piscinibacter sakaiensis (bacteria)
KeywordsHYDROLASE / PET hydrolase / PET degradation enzyme

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