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TitleIn situ insights into antibody-mediated neutralization of a pre-fusion Junin virus glycoprotein complex.
Journal, issue, pagesCell Rep, Vol. 44, Issue 7, Page 115971, Year 2025
Publish dateJul 8, 2025
AuthorsLily J Taylor / Michael R Sawaya / Jonna B Westover / Chenyi Wang / Frederick Jimenez / Aldo J Muñoz / Julian Whitelegge / Brian B Gowen / Gustavo F Helguera / Roger Castells-Graells / Jose A Rodriguez /
PubMed AbstractA transmembrane glycoprotein complex (GPC) decorates the Junin mammarenavirus (JUNV) that causes New World hemorrhagic fevers. We leveraged single-particle cryoelectron microscopy (cryo-EM) to image ...A transmembrane glycoprotein complex (GPC) decorates the Junin mammarenavirus (JUNV) that causes New World hemorrhagic fevers. We leveraged single-particle cryoelectron microscopy (cryo-EM) to image the full-length JUNV GPC directly on pseudotyped virus (PV) membranes and bound by two JUNV-neutralizing antibodies: Candid#1 vaccine-elicited CR1-28 and J199, a potent therapeutic against Argentine hemorrhagic fever (AHF). The 3.8 Å resolution in situ structures of the antibody-neutralized, 3-fold symmetric JUNV GPC reveal its ectodomain architecture, signal peptide-bound transmembrane region, zinc-binding luminal domain, and post-translational modifications. JUNV-GPC sequence variants highlight the functional importance of the signal peptide transmembrane helix register for virus infection and attenuating Candid#1-associated variants. Overlapping CR1-28 and J199 epitopes suggest a common receptor-blocking mechanism for JUNV neutralization, while a J199-induced, symmetric GPC reorientation may further drive its potent inhibition of JUNV lethality in mice, compared to receptor blockade alone. This underscores the utility of in situ insights into GPC function and neutralization.
External linksCell Rep / PubMed:40632652
MethodsEM (single particle)
Resolution3.8 Å
Structure data

48221

9mew

EMDB-48221, PDB-9mew:
JUNV GP1, GP2, SSP and CR1-28 Fab complex in a pseudotyped virus membrane
Method: EM (single particle) / Resolution: 3.8 Å

48781

9n0d

EMDB-48781, PDB-9n0d:
JUNV GP1, GP2, SSP complex with neutralizing antibody in a pseudotyped virus membrane
Method: EM (single particle) / Resolution: 3.8 Å

Chemicals

ChemComp-MYR:
MYRISTIC ACID

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

Source
  • mammarenavirus juninense
  • homo sapiens (human)
  • mus musculus (house mouse)
KeywordsVIRAL PROTEIN/IMMUNE SYSTEM / Viral protein / Glycoprotein / GPC / JUNV / Junin mammarenavirus / GP1 / GP2 / signal peptide / virus membrane / CR1-28 Fab / VIRAL PROTEIN-IMMUNE SYSTEM complex / antibody

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