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Structure paper

TitleStructure of a type II SMC Wadjet complex from Neobacillus vireti.
Journal, issue, pagesStructure, Vol. 33, Issue 9, Page 1498-11509.e5, Year 2025
Publish dateSep 4, 2025
AuthorsFlorian Roisné-Hamelin / Hon Wing Liu / Stephan Gruber /
PubMed AbstractStructural maintenance of chromosome complexes are essential DNA-folding motors that facilitate critical cellular functions, including chromosome segregation and DNA repair. Wadjet systems are ...Structural maintenance of chromosome complexes are essential DNA-folding motors that facilitate critical cellular functions, including chromosome segregation and DNA repair. Wadjet systems are prokaryotic SMC complexes specialized in cellular immunity against plasmids. Type I Wadjet systems restrict plasmids via a DNA extrusion-cleavage reaction. Two other Wadjet types (II and III) have also been identified, however, their molecular characteristics are unclear. Here, we reconstituted a representative type II Wadjet system from Neobacillus vireti. We show that this system shares substrate selection and cleavage properties with type I but exhibits distinctive structural features, including a long elbow-distal coiled coil, a channel-less hinge, and a tandem KITE subunit. These features help identify the common and distinguishing architectural elements in the family of Wadjet systems and raise intriguing questions about the evolution of prokaryotic SMC complexes.
External linksStructure / PubMed:40628265
MethodsEM (single particle)
Resolution3.5 - 6.71 Å
Structure data

53035

9qe0

EMDB-53035, PDB-9qe0:
Neobacillus vireti Wadjet-II JetABC dimer
Method: EM (single particle) / Resolution: 6.71 Å

EMDB-53036: Neobacillus vireti Wadjet-II JetC hinge
Method: EM (single particle) / Resolution: 4.38 Å

53037

9qe1

EMDB-53037, PDB-9qe1:
Neobacillus vireti Wadjet-II JetABC monomer
Method: EM (single particle) / Resolution: 3.5 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

Source
  • neobacillus vireti lmg 21834 (bacteria)
KeywordsDNA BINDING PROTEIN / SMC complexes / Wadjet / JetABCD / DNA loop extrusion

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