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TitleStructure of a transcribing Pol II-DSIF-SPT6-U1 snRNP complex.
Journal, issue, pagesNat Commun, Vol. 16, Issue 1, Page 5823, Year 2025
Publish dateJul 1, 2025
AuthorsLuojia Zhang / Christopher Batters / Shintaro Aibara / Yuliya Gordiyenko / Kristina Žumer / Jana Schmitzová / Kerstin Maier / Patrick Cramer / Suyang Zhang /
PubMed AbstractIn eukaryotic cells, splicing occurs predominantly co-transcriptionally, enhancing splicing efficiency and fidelity while introducing an additional layer of regulation over gene expression. RNA ...In eukaryotic cells, splicing occurs predominantly co-transcriptionally, enhancing splicing efficiency and fidelity while introducing an additional layer of regulation over gene expression. RNA polymerase II (Pol II) facilitates co-transcriptional splicing by recruiting the U1 small nuclear ribonucleoprotein particle (U1 snRNP) to the nascent transcripts. Here, we report the cryo-electron microscopy structure of a transcribing Pol II-U1 snRNP complex with elongation factors DSIF and SPT6. In addition, our biochemical analysis reveals that the phosphorylated Pol II carboxyl-terminal domain and SPT6 interact directly with U1 snRNP proteins, facilitating its recruitment to the elongation complex. This multivalent interaction between U1 snRNP and the transcription elongation complex may both allow efficient spliceosome assembly and ensure transcription processivity.
External linksNat Commun / PubMed:40595577 / PubMed Central
MethodsEM (single particle)
Resolution3.5 - 7.2 Å
Structure data

53087

9qeq

EMDB-53087: Overall map of the transcribing Pol II-DSIF-SPT6-U1 snRNP complex
PDB-9qeq: Structure of the transcribing Pol II-DSIF-SPT6-U1 snRNP complex
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-53088: Focused refined map of SPT6 in the EC-DSIF-SPT6-U1 snRNP complex
Method: EM (single particle) / Resolution: 6.2 Å

EMDB-53089: Focused refined map of U1 snRNP in the EC-DSIF-SPT6-U1 snRNP complex
Method: EM (single particle) / Resolution: 7.2 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

Source
  • homo sapiens (human)
  • sus scrofa domesticus (domestic pig)
  • synthetic construct (others)
KeywordsSPLICING / Transcription / elongation / cryo-EM

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