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TitleCryoEM structure of the SLFN14 endoribonuclease reveals insight into RNA binding and cleavage.
Journal, issue, pagesNat Commun, Vol. 16, Issue 1, Page 5848, Year 2025
Publish dateJul 1, 2025
AuthorsJustin Van Riper / Arleth O Martinez-Claros / Lie Wang / Hannah E Schneiderman / Sweta Maheshwari / Monica C Pillon /
PubMed AbstractThe SLFN14 endoribonuclease is a post-transcriptional regulator that targets the ribosome and its associated RNA substrates for codon-bias translational repression. SLFN14 nuclease activity is linked ...The SLFN14 endoribonuclease is a post-transcriptional regulator that targets the ribosome and its associated RNA substrates for codon-bias translational repression. SLFN14 nuclease activity is linked to antiviral defense and platelet function. Despite its prominent role in gene regulation, the molecular signals regulating SLFN14 substrate recognition and catalytic activation remain unclear. SLFN14 dysregulation is linked to human diseases, including ribosomopathies and inherited thrombocytopenia, thus underscoring the importance of establishing the signals coordinating its RNA processing activity. Here, we reconstitute active full-length human SLFN14 and report a high-resolution cryoEM reconstruction of the SLFN14•RNA complex. The structure reveals a medallion-like architecture that shares structural homology with other SLFN family members. We unveil a C-terminal hydrophobic intermolecular interface that stabilizes the SLFN14 homodimer without the need for additional molecular signals. We describe compact sequence-independent RNA binding interfaces and highlight the environment of the SLFN14 disease hotspot at the RNA cleft entrance. We show that the SLFN14 endoribonuclease has broad site-specificity in the absence of modified native tRNA, a characteristic not shared with its SLFN11 family member. Finally, we demonstrate that metal-dependent acceptor stem cleavage requires the SLFN14 E-EhK motif and uncover its unexpected parallel with other virus-activatable nucleases.
External linksNat Commun / PubMed:40592880 / PubMed Central
MethodsEM (single particle)
Resolution2.73 - 3.11 Å
Structure data

49946

9nyy

EMDB-49946, PDB-9nyy:
Nucleic acid bound human SLFN14, State 1
Method: EM (single particle) / Resolution: 2.73 Å

EMDB-49947: Nucleic acid bound human SLFN14, State 2
Method: EM (single particle) / Resolution: 3.11 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

Source
  • homo sapiens (human)
KeywordsRNA BINDING PROTEIN / nuclease / antiviral factor / post-transcriptional regulator

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