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TitleCharacterization of the Structure and Function of the Photosynthetic RC-LH1 Core Supercomplex From Rhodospirillum rubrum.
Journal, issue, pagesPhysiol Plant, Vol. 177, Issue 3, Page e70275, Year 2025
Publish dateMay 19, 2025
AuthorsBern Christianson / Zekun Liu / Yingyue Zhang / Chen Wang / Adrian M Gardner / Yu-Zhong Zhang / Peng Wang / Lu-Ning Liu /
PubMed AbstractPhotosynthetic reaction center-light harvesting 1 (RC-LH1) core supercomplexes are essential for energy capture and electron transport in purple bacteria. Rhodospirillum rubrum, a model organism for ...Photosynthetic reaction center-light harvesting 1 (RC-LH1) core supercomplexes are essential for energy capture and electron transport in purple bacteria. Rhodospirillum rubrum, a model organism for bacterial photosynthesis, features an RC-LH1 architecture with a closed LH1 ring and lacks the peripheral LH2 antenna in the photosynthetic membranes. How this unique RC-LH1 supercomplex performs energy transfer and quinone transport remains unclear. Here, we characterized both the structural and functional properties of Rsp. rubrum RC-LH1 supercomplex using cryo-electron microscopy (cryo-EM), transient absorption (TA) spectroscopy, and cytochrome c oxidation assays. Cryo-EM of the RC-LH1 monomeric structure revealed a closed LH1 ring of 16 αβ-polypeptides encircling the RC, with weaker RC-LH1 interactions than other RC-LH1 structures reported. TA spectra and cytochrome c oxidation assays showed that Rsp. rubrum RC-LH1 monomer with a closed LH1 ring exhibits slower and more distributed excitation energy transfer (EET) kinetics from LH1 to RC and slower electron transport rates than Rba. sphaeroides RC-LH1 monomer with a large opening in the LH1 ring. Our findings provide insight into the unique architecture and spectroscopic properties of Rsp. rubrum RC-LH1 supercomplex. This study enhances our understanding of bacterial photosynthetic mechanisms and lays the foundation for bioengineering applications in artificial photosynthetic systems.
External linksPhysiol Plant / PubMed:40384483 / PubMed Central
MethodsEM (single particle)
Resolution3.02 Å
Structure data

62025

9k3q

EMDB-62025, PDB-9k3q:
Cryo-EM structure of the Rhodospirillum rubrum RC-LH1 complex
Method: EM (single particle) / Resolution: 3.02 Å

Chemicals

ChemComp-07D:
Trans-Geranyl BACTERIOCHLOROPHYLL A

ChemComp-CRT:
SPIRILLOXANTHIN

ChemComp-FE:
Unknown entry

ChemComp-BPH:
BACTERIOPHEOPHYTIN A

ChemComp-U10:
UBIQUINONE-10

Source
  • rhodospirillum rubrum (bacteria)
KeywordsPHOTOSYNTHESIS / reaction centre light-harvesting 1

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