Title Capturing structural intermediates in an animal-like cryptochrome photoreceptor by time-resolved crystallography. Journal, issue, pages Sci Adv , Vol. 11, Page eadu7247-eadu7247, Year 2025Publish date Apr 11, 2024 (structure data deposition date) AuthorsMaestre-Reyna, M. / Hosokawa, Y. / Wang, P.H. / Saft, M. / Caramello, N. / Engilberge, S. / Franz-Badur, S. / Gusti Ngurah Putu, E.P. / Nakamura, M. / Wu, W.J. ...Maestre-Reyna, M. / Hosokawa, Y. / Wang, P.H. / Saft, M. / Caramello, N. / Engilberge, S. / Franz-Badur, S. / Gusti Ngurah Putu, E.P. / Nakamura, M. / Wu, W.J. / Wu, H.Y. / Lee, C.C. / Huang, W.C. / Huang, K.F. / Chang, Y.K. / Yang, C.H. / Fong, M.I. / Lin, W.T. / Yang, K.C. / Ban, Y. / Imura, T. / Kazuoka, A. / Tanida, E. / Owada, S. / Joti, Y. / Tanaka, R. / Tanaka, T. / Kang, J. / Luo, F. / Tono, K. / Kiontke, S. / Korf, L. / Umena, Y. / Tosha, T. / Bessho, Y. / Nango, E. / Iwata, S. / Royant, A. / Tsai, M.D. / Yamamoto, J. / Essen, L.O. External links Sci Adv / PubMed:40378212Methods X-ray diffraction Resolution 1.64 - 2.2 Å Structure data PDB-8z1j : Structure viewer SFX structure of dark adapted CraCRY Method : X-RAY DIFFRACTION / Resolution : 1.64 Å
PDB-8z24 : Structure viewer SFX structure of CraCRY 10 ns after photoexcitation of the oxidized proteinMethod : X-RAY DIFFRACTION / Resolution : 1.95 Å
PDB-8z26 : Structure viewer SFX structure of CraCRY 30 ns after photoexcitation of the oxidized proteinMethod : X-RAY DIFFRACTION / Resolution : 2 Å
PDB-8z2d : Structure viewer SFX structure of CraCRY 100 ns after photoexcitation of the oxidized proteinMethod : X-RAY DIFFRACTION / Resolution : 2 Å
PDB-8z3d : Structure viewer SFX structure of CraCRY 1 us after photoexcitation of the oxidized proteinMethod : X-RAY DIFFRACTION / Resolution : 2 Å
PDB-8z3g : Structure viewer SFX structure of CraCRY 3 us after photoexcitation of the oxidized proteinMethod : X-RAY DIFFRACTION / Resolution : 1.95 Å
PDB-8z3l : Structure viewer SFX structure of CraCRY 10 us after photoexcitation of the oxidized proteinMethod : X-RAY DIFFRACTION / Resolution : 2 Å
PDB-8z3x : Structure viewer SFX structure of CraCRY 30 us after photoexcitation of the oxidized proteinMethod : X-RAY DIFFRACTION / Resolution : 1.95 Å
PDB-8z41 : Structure viewer SFX structure of CraCRY 100 us after photoexcitation of the oxidized proteinMethod : X-RAY DIFFRACTION / Resolution : 1.95 Å
PDB-8z44 : Structure viewer SFX structure of CraCRY 300 ns after photoexcitation of the oxidized proteinMethod : X-RAY DIFFRACTION / Resolution : 2 Å
PDB-8z45 : Structure viewer SFX structure of CraCRY 300 us after photoexcitation of the oxidized proteinMethod : X-RAY DIFFRACTION / Resolution : 2 Å
PDB-8z4k : Structure viewer SFX structure of CraCRY 1 ms after photoexcitation of the oxidized proteinMethod : X-RAY DIFFRACTION / Resolution : 2 Å
PDB-8z4m : Structure viewer SFX structure of CraCRY 7 ms after photoexcitation of the oxidized proteinMethod : X-RAY DIFFRACTION / Resolution : 1.9 Å
PDB-8z4p : Structure viewer SFX structure of CraCRY 33 ms after photoexcitation of the oxidized proteinMethod : X-RAY DIFFRACTION / Resolution : 2.05 Å
PDB-8z4u : Structure viewer SFX structure of CraCRY 66 ms after photoexcitation of the oxidized proteinMethod : X-RAY DIFFRACTION / Resolution : 2 Å
PDB-8z6i : Structure viewer SFX structure of CraCRY 100 ms after photoexcitation of the oxidized proteinMethod : X-RAY DIFFRACTION / Resolution : 1.9 Å
PDB-8z6j : Structure viewer SFX structure of CraCRY 133 ms after photoexcitation of the oxidized proteinMethod : X-RAY DIFFRACTION / Resolution : 2 Å
PDB-8z6k : Structure viewer SFX structure of CraCRY 166 ms after photoexcitation of the oxidized proteinMethod : X-RAY DIFFRACTION / Resolution : 2.2 Å
PDB-8z8f : Structure viewer SFX structure of CraCRY 200 ms after photoexcitation of the oxidized proteinMethod : X-RAY DIFFRACTION / Resolution : 2.15 Å
PDB-8z8k : Structure viewer SFX structure of CraCRY 233 ms after photoexcitation of the oxidized proteinMethod : X-RAY DIFFRACTION / Resolution : 2.2 Å
PDB-8za8 : Structure viewer SFX structure of CraCRY in the fully reduced stateMethod : X-RAY DIFFRACTION / Resolution : 2.1 Å
PDB-8zlr : Structure viewer SFX structure of CraCRY in the semiquinone stateMethod : X-RAY DIFFRACTION / Resolution : 2.15 Å
Chemicals ChemComp-FAD : Structure viewer FLAVIN-ADENINE DINUCLEOTIDE / FAD*YM
ChemComp-FDA : Structure viewer DIHYDROFLAVINE-ADENINE DINUCLEOTIDE
Source chlamydomonas reinhardtii cc3269 (plant)chlamydomonas reinhardtii (plant) Keywords FLAVOPROTEIN / photoreceptor / time-resolved crystallography / resting state / 10 ns time-point / 30 ns time-point / 100 ns time-point / 1 us time-point / 3 us time-point / 10 us time-point / 30 us time-point / 100 us time-point / 300 ns time-point / 300 us time-point / 1 ms time-point / 7 ms time-point / 33 ms time-point / 66 ms time-point / 100 ms time-point / 133 ms time-point / 166 ms time-point / 200 ms time-point / 233 ms time-point / fully reduced state / semiquinone state