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Structure paper

TitleMechanism and application of thiol-disulfide redox biosensors with a fluorescence-lifetime readout.
Journal, issue, pagesProc. Natl. Acad. Sci. USA, Vol. 122, Page e2503978122-e2503978122, Year 2025
Publish dateJan 14, 2025 (structure data deposition date)
AuthorsRosen, P.C. / Glaser, A. / Martinez-Francois, J.R. / Lim, D.C. / Brooks, D.J. / Fu, P. / Kim, E. / Kern, D. / Yellen, G.
External linksProc. Natl. Acad. Sci. USA / PubMed:40327692
MethodsX-ray diffraction
Resolution2 - 2.2 Å
Structure data

PDB-9mus:
Reduced state of a turn-off thiol-disulfide redox biosensor with a fluorescence-lifetime readout
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-9mut:
Reduced state of a turn-on thiol-disulfide redox biosensor with a fluorescence-lifetime readout
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-9muu:
Oxidized state of a turn-off thiol-disulfide redox biosensor with a fluorescence-lifetime readout
Method: X-RAY DIFFRACTION / Resolution: 2.15 Å

PDB-9muv:
Oxidized state of a turn-on thiol-disulfide redox biosensor with a fluorescence-lifetime readout
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

Chemicals

ChemComp-ACY:
ACETIC ACID

ChemComp-GOL:
GLYCEROL

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-HOH:
WATER

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-ACT:
ACETATE ION

ChemComp-FMT:
FORMIC ACID

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

Source
  • aequorea victoria (jellyfish)
KeywordsFLUORESCENT PROTEIN / fluorescence lifetime / thiol-disulfide redox

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