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| Title | Asymmetric structure of podophage N4 from the Schitoviridae family reveals a type of tube-sheath short-tail architecture. |
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| Journal, issue, pages | Structure, Vol. 33, Issue 7, Page 1250-11262.e2, Year 2025 |
| Publish date | Jul 3, 2025 |
 Authors | Jing Zheng / Hao Pang / Hao Xiao / Junquan Zhou / Zhonghua Liu / Wenyuan Chen / Hongrong Liu /  |
| PubMed Abstract | The tails of the majority of reported podophages are typically composed of an adaptor, a nozzle, and a needle, and flanked by six or twelve fibers. However, the Schitoviridae family, as represented ...The tails of the majority of reported podophages are typically composed of an adaptor, a nozzle, and a needle, and flanked by six or twelve fibers. However, the Schitoviridae family, as represented by podophage N4, exhibits a different tail architecture that remains poorly understood. In this study, we employed cryoelectron microscopy (cryo-EM) to determine the atomic structures of mature and empty podophage N4 particles. The N4 tail, which is connected to the head by a portal and flanked by 12 fibers, comprises an adaptor, a 12-fold extended tail tube encircled by a 6-fold tail sheath, and a plug. The extended tail sheath is composed of two proteins, gp65 and gp64. Furthermore, we identified two distinct tail conformations in the mature podophage N4. Our structures provide insights into the mechanisms of ejection and early transcription of podophage N4, as well as for N4-like phages and CrAssphages. |
 External links | Structure / PubMed:40318628 |
| Methods | EM (single particle) |
| Resolution | 2.4 - 23.8 Å |
| Structure data | EMDB-62959, PDB-9lbz:  EMDB-62960: Conformation B of unique vertex of mature phage N4 EMDB-62961, PDB-9lc0: EMDB-62962, PDB-9lc1:  EMDB-62963: Distal tip of mature phage N4  EMDB-62964: portal tail of empty phage N4  EMDB-62965: E-hexon of mature phage N4  EMDB-62966: 3-fold region of mature phage N4  EMDB-62967: 5-fold region of mature phage N4  EMDB-62968: mismatch reconstruction of empty phage N4  EMDB-62969: Comformation B of mature phage N4  EMDB-62970: Conformation A of mature phage N4 EMDB-63001, PDB-9ld7: |
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 Keywords | VIRAL PROTEIN / Complex / VIRUS |
escherichia phage n4 (virus)