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Structure paper

TitleBacterial pathogen deploys the iminosugar glycosyrin to manipulate plant glycobiology.
Journal, issue, pagesScience, Vol. 388, Issue 6744, Page 297-303, Year 2025
Publish dateApr 18, 2025
AuthorsNattapong Sanguankiattichai / Balakumaran Chandrasekar / Yuewen Sheng / Nathan Hardenbrook / Werner W A Tabak / Margit Drapal / Farnusch Kaschani / Clemens Grünwald-Gruber / Daniel Krahn / Pierre Buscaill / Suzuka Yamamoto / Atsushi Kato / Robert Nash / George Fleet / Richard Strasser / Paul D Fraser / Markus Kaiser / Peijun Zhang / Gail M Preston / Renier A L van der Hoorn /
PubMed AbstractThe extracellular space (apoplast) in plants is a key battleground during microbial infections. To avoid recognition, the bacterial model phytopathogen pv. DC3000 produces glycosyrin. Glycosyrin ...The extracellular space (apoplast) in plants is a key battleground during microbial infections. To avoid recognition, the bacterial model phytopathogen pv. DC3000 produces glycosyrin. Glycosyrin inhibits the plant-secreted β-galactosidase BGAL1, which would otherwise initiate the release of immunogenic peptides from bacterial flagellin. Here, we report the structure, biosynthesis, and multifunctional roles of glycosyrin. High-resolution cryo-electron microscopy and chemical synthesis revealed that glycosyrin is an iminosugar with a five-membered pyrrolidine ring and a hydrated aldehyde that mimics monosaccharides. Glycosyrin biosynthesis was controlled by virulence regulators, and its production is common in bacteria and prevents flagellin recognition and alters the extracellular glycoproteome and metabolome of infected plants. These findings highlight a potentially wider role for glycobiology manipulation by plant pathogens across the plant kingdom.
External linksScience / PubMed:40245141 / PubMed Central
MethodsEM (single particle)
Resolution1.42 - 2.06 Å
Structure data

19181

8ri6

EMDB-19181, PDB-8ri6:
Beta-galactosidase (LacZ) purified from E. coli.
Method: EM (single particle) / Resolution: 2.06 Å

19182

8ri7

EMDB-19182, PDB-8ri7:
Beta-galactosidase (LacZ) in complex with glycosyrin from Pseudomonas syringae.
Method: EM (single particle) / Resolution: 1.93 Å

19183

8ri8

EMDB-19183, PDB-8ri8:
Beta-galactosidase (LacZ) in complex with synthetic glycosyrin.
Method: EM (single particle) / Resolution: 1.42 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-NA:
Unknown entry

ChemComp-HOH:
WATER

PDB-1h05:
3-DEHYDROQUINATE DEHYDRATASE FROM MYCOBACTERIUM TUBERCULOSIS IN COMPLEX WITH SULPHATE

Source
  • escherichia coli (E. coli)
KeywordsHYDROLASE / beta-galactosidase / inhibitor / iminosugar / plant pathogenic bacteria.

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