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Structure paper

TitleStructural Basis of Sequential Enantioselective Epoxidation by a Flavin-Dependent Monooxygenase in Lasalocid A Biosynthesis.
Journal, issue, pagesAngew. Chem. Int. Ed. Engl., Vol. 64, Page e202504982-e202504982, Year 2025
Publish dateOct 20, 2023 (structure data deposition date)
AuthorsWang, Q. / Deng, Y. / Viera, D. / Liu, X. / Liu, N. / Hu, Y. / Hu, X. / Wei, H. / Zhou, Q. / Lan, T. ...Wang, Q. / Deng, Y. / Viera, D. / Liu, X. / Liu, N. / Hu, Y. / Hu, X. / Wei, H. / Zhou, Q. / Lan, T. / He, W. / Chen, X. / Kim, C.Y.
External linksAngew. Chem. Int. Ed. Engl. / PubMed:40199722
MethodsX-ray diffraction
Resolution1.51 - 2.2 Å
Structure data

PDB-8up4:
Crystal structure of Lsd18 (a flavin-dependent monooxygenase)
Method: X-RAY DIFFRACTION / Resolution: 1.51 Å

PDB-8xtz:
Crystal structure of Lsd18 in complex with a substrate
Method: X-RAY DIFFRACTION / Resolution: 1.85 Å

PDB-8xu7:
Crystal structure of Lsd18 in complex with a product
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

Chemicals

ChemComp-FAD:
FLAVIN-ADENINE DINUCLEOTIDE / FAD*YM

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-DMS:
DIMETHYL SULFOXIDE / DMSO, precipitant*YM

PDB-1lwm:
Solution Structure of the Sequence-Non-Specific HMGB protein NHP6A

ChemComp-LSB:
(4R,5S)-3-((2R,3S,4S)-2-ethyl-5-((2R,3R)-2-ethyl-3-(2-((2R,3R)-2-ethyl-3-methyloxiran-2-yl)ethyl)oxiran-2-yl)-3-hydroxy-4-methylpentanoyl)-4-methyl-5-phenyloxazolidin-2-one

Source
  • streptomyces lasalocidi (bacteria)
KeywordsFLAVOPROTEIN / a flavin-dependent monooxygenase / epoxidase / FAD-dependent monooxygenase / flavin-dependent monooxygenase / epoxidation

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