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TitleDimerization of GAS2 mediates crosslinking of microtubules and F-actin.
Journal, issue, pagesEMBO J, Vol. 44, Issue 10, Page 2997-3024, Year 2025
Publish dateApr 1, 2025
AuthorsJiancheng An / Tsuyoshi Imasaki / Akihiro Narita / Shinsuke Niwa / Ryohei Sasaki / Tsukasa Makino / Ryo Nitta / Masahide Kikkawa /
PubMed AbstractThe spectraplakin family protein GAS2 was originally identified as a growth arrest-specific protein, and recent studies have revealed its involvement in multiple cellular processes. Its dual ...The spectraplakin family protein GAS2 was originally identified as a growth arrest-specific protein, and recent studies have revealed its involvement in multiple cellular processes. Its dual interaction with actin filaments and microtubules highlights its essential role in cytoskeletal organization, such as cell division, apoptosis, and possibly tumorigenesis. However, the structural basis of cytoskeletal dynamics regulation by GAS2 remains unclear. In this study, we present cryo-electron microscopy structures of the GAS2 type 3 calponin homology domain (CH3) in complex with F-actin at 2.8 Å resolution, thus solving the first type CH3 domain structure bound to F-actin and confirming its actin-binding activity. We also provide the first near-atomic resolution cryo-EM structure of the GAS2-GAR domain bound to microtubules and identify conserved microtubule-binding residues. Our biochemical experiments show that GAS2 promotes microtubule nucleation and polymerization, and that its C-terminal region is essential for dimerization, bundling of both F-actin and microtubules, and microtubule nucleation. As mutations leading to expression of C-terminally truncated GAS2 have been linked to hearing loss, these findings suggest that the disruption of GAS2-dependent cytoskeletal organisation could underlie auditory dysfunction.
External linksEMBO J / PubMed:40169809 / PubMed Central
MethodsEM (helical sym.)
Resolution2.8 - 3.2 Å
Structure data

62232

9kbw

EMDB-62232, PDB-9kbw:
CryoEM structure of microtubule bound with GAS2-GAR domain.
Method: EM (helical sym.) / Resolution: 3.2 Å

62233

9kbx

EMDB-62233, PDB-9kbx:
CryoEM structure of F-actin bound with GAS2-CH3 domain.
Method: EM (helical sym.) / Resolution: 2.8 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

Source
  • mus musculus (house mouse)
  • sus scrofa (pig)
  • oryctolagus cuniculus (rabbit)
KeywordsSTRUCTURAL PROTEIN / Cytoskeleton

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