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Structure paper

TitleStructural Plasticity of Flavin-Dependent Thymidylate Synthase Controlled by the Enzyme Redox State.
Journal, issue, pagesBiomolecules, Vol. 15, Year 2025
Publish dateDec 12, 2023 (structure data deposition date)
AuthorsPecqueur, L. / Lombard, M. / Hamdane, D.
External linksBiomolecules / PubMed:40149854
MethodsX-ray diffraction
Resolution1.94 - 2.201 Å
Structure data

PDB-8ren:
Crystal structure of reduced ThyX
Method: X-RAY DIFFRACTION / Resolution: 2.139 Å

PDB-8reo:
Crystal structure of reduced ThyX in complex with dUMP
Method: X-RAY DIFFRACTION / Resolution: 2.03 Å

PDB-8rep:
Crystal structure of oxidized ThyX-Y91F mutant
Method: X-RAY DIFFRACTION / Resolution: 2.201 Å

PDB-8req:
Crystal structure of reduced ThyX-Y91F mutant
Method: X-RAY DIFFRACTION / Resolution: 1.94 Å

Chemicals

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

ChemComp-FDA:
DIHYDROFLAVINE-ADENINE DINUCLEOTIDE

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-HOH:
WATER

ChemComp-UMP:
2'-DEOXYURIDINE 5'-MONOPHOSPHATE

ChemComp-P6G:
HEXAETHYLENE GLYCOL / precipitant*YM

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-FAD:
FLAVIN-ADENINE DINUCLEOTIDE / FAD*YM

ChemComp-PG6:
1-(2-METHOXY-ETHOXY)-2-{2-[2-(2-METHOXY-ETHOXY]-ETHOXY}-ETHANE

Source
  • thermotoga maritima (bacteria)
KeywordsTRANSFERASE / FLAVIN-DEPENDEN THYMIDYLATE SYNTHASE / METHYLENETETRAHYDROFOLATE

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