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TitleCryo-EM Analysis of a Unique Subnucleosome Containing Centromere-Specific Histone Variant CENP-A.
Journal, issue, pagesGenes Cells, Vol. 30, Issue 2, Page e70016, Year 2025
Publish dateMay 14, 2025
AuthorsOsamu Kawasaki / Yoshimasa Takizawa / Iori Kiyokawa / Hitoshi Kurumizaka / Kayo Nozawa /
PubMed AbstractIn eukaryotes, genomic DNA is stored in the nucleus as nucleosomes, in which a DNA segment is wrapped around a protein octamer consisting of two each of the four histones, H2A, H2B, H3, and H4. The ...In eukaryotes, genomic DNA is stored in the nucleus as nucleosomes, in which a DNA segment is wrapped around a protein octamer consisting of two each of the four histones, H2A, H2B, H3, and H4. The core histones can be replaced by histone variants or altered with covalent modifications, contributing to the regulation of chromosome structure and nuclear activities. The formation of an octameric histone core in nucleosomes is widely accepted. Recently, the H3-H4 octasome, a novel nucleosome-like structure with a histone octamer consisting solely of H3 and H4, has been reported. CENP-A is the centromere-specific histone H3 variant and determines the position of kinetochore assembly during mitosis. CENP-A is a distant H3 variant sharing approximately 50% amino acid sequence with H3. In this study, we found that CENP-A and H4 also formed an octamer without H2A and H2B in vitro. We determined the structure of the CENP-A-H4 octasome at 3.66 Å resolution. In the CENP-A-H4 octasome, an approximately 120-base pair DNA segment was wrapped around the CENP-A-H4 octameric core and displayed the four CENP-A RG-loops, which are the direct binding sites for another centromeric protein, CENP-N.
External linksGenes Cells / PubMed:40129080 / PubMed Central
MethodsEM (single particle)
Resolution3.66 Å
Structure data

62193

9k9l

EMDB-62193, PDB-9k9l:
Cryo-EM structure of the human CENP-A-H4 octasome.
Method: EM (single particle) / Resolution: 3.66 Å

Source
  • homo sapiens (human)
  • synthetic construct (others)
KeywordsDNA BINDING PROTEIN/DNA / subnucleosome / NUCLEAR PROTEIN / DNA BINDING PROTEIN-DNA complex / DNA BINDING PROTEIN

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