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TitleStructural recognition and stabilization of tyrosine hydroxylase by the J-domain protein DNAJC12.
Journal, issue, pagesNat Commun, Vol. 16, Issue 1, Page 2755, Year 2025
Publish dateMar 20, 2025
AuthorsMary Dayne S Tai / Lissette Ochoa / Marte I Flydal / Lorea Velasco-Carneros / Jimena Muntaner / César Santiago / Gloria Gamiz-Arco / Fernando Moro / Kunwar Jung-Kc / David Gil-Cantero / Miguel Marcilla / Juha P Kallio / Arturo Muga / José María Valpuesta / Jorge Cuéllar / Aurora Martinez /
PubMed AbstractPathogenic variants of the J-domain protein DNAJC12 cause parkinsonism, which is associated with a defective interaction of DNAJC12 with tyrosine hydroxylase (TH), the rate-limiting enzyme in ...Pathogenic variants of the J-domain protein DNAJC12 cause parkinsonism, which is associated with a defective interaction of DNAJC12 with tyrosine hydroxylase (TH), the rate-limiting enzyme in dopamine biosynthesis. In this work, we characterize the formation of the TH:DNAJC12 complex, showing that DNAJC12 binding stabilizes both TH and the variant TH-p.R202H, associated with TH deficiency. This binding delays their time-dependent aggregation in an Hsp70-independent manner, while preserving TH activity and feedback regulatory inhibition by dopamine. DNAJC12 alone barely activates Hsc70 but synergistically stimulates Hsc70 ATPase activity when complexed with TH. Cryo-electron microscopy supported by crosslinking-mass spectroscopy reveals two DNAJC12 monomers bound per TH tetramer, each embracing one of the two regulatory domain dimers, leaving the active sites available for substrate, cofactor and inhibitory dopamine interaction. Our results also reveal the key role of the C-terminal region of DNAJC12 in TH binding, explaining the pathogenic mechanism of the DNAJC12 disease variant p.W175Ter.
External linksNat Commun / PubMed:40113792 / PubMed Central
MethodsEM (single particle)
Resolution4.45 - 5.3 Å
Structure data

EMDB-18047: 3D Reconstruction of TH-DNAJC12 WT Complex
Method: EM (single particle) / Resolution: 4.45 Å

EMDB-18058: TH-DNAJC12 (108-198)
Method: EM (single particle) / Resolution: 5.05 Å

EMDB-18289: TH(DA)-DNAJC12 Complex 3D reconstruction
Method: EM (single particle) / Resolution: 5.3 Å

Source
  • Homo sapiens (human)

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