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TitleCharacterization and Engineering of a Bisabolene Synthase Reveal an Unusual Hydride Shift and Key Residues Critical for Mono-, Bi-, and Tricyclic Sesquiterpenes Formation.
Journal, issue, pagesJ. Am. Chem. Soc., Vol. 147, Page 10413-10422, Year 2025
Publish dateApr 8, 2024 (structure data deposition date)
AuthorsWu, M. / Torrence, I. / Liu, Y. / Wu, J. / Ge, R. / Ma, K. / Liu, D. / Ren, J. / Fan, S. / Ma, M. ...Wu, M. / Torrence, I. / Liu, Y. / Wu, J. / Ge, R. / Ma, K. / Liu, D. / Ren, J. / Fan, S. / Ma, M. / Siegel, J.B. / Tantillo, D.J. / Lin, W. / Fan, A.
External linksJ. Am. Chem. Soc. / PubMed:40071547
MethodsX-ray diffraction
Resolution1.73 - 3.8 Å
Structure data

PDB-8yzu:
STC truncated SydA
Method: X-RAY DIFFRACTION / Resolution: 2.01 Å

PDB-8yzx:
Sesquiterpene synthase SydA
Method: X-RAY DIFFRACTION / Resolution: 3.8 Å

PDB-8yzy:
STC SydA mutant - W297Y
Method: X-RAY DIFFRACTION / Resolution: 1.73 Å

Chemicals

ChemComp-POP:
PYROPHOSPHATE 2-

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

Source
  • aspergillus versicolor (mold)
KeywordsBIOSYNTHETIC PROTEIN / sesquiterpene synthase / sesquiterpene synthase mutant

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