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TitleCryo-EM structure of the MexY multidrug efflux pump.
Journal, issue, pagesmBio, Vol. 16, Issue 4, Page e0382624, Year 2025
Publish dateApr 9, 2025
AuthorsWilliam D Gregor / Rakesh Maharjan / Zhemin Zhang / Lucius Chiaraviglio / Nithya Sastry / Meng Cui / James E Kirby / Edward W Yu /
PubMed Abstract, a Gram-negative pathogen, has emerged as one of the most highly antibiotic-resistant bacteria worldwide and subsequently has become a leading cause of healthcare-associated, life-threatening ..., a Gram-negative pathogen, has emerged as one of the most highly antibiotic-resistant bacteria worldwide and subsequently has become a leading cause of healthcare-associated, life-threatening infections. multidrug efflux Y (MexY) is an efflux pump that belongs to the resistance-nodulation-cell division (RND) superfamily. It is a major determinant for resistance to aminoglycosides in this opportunistic pathogen. However, the detailed molecular mechanisms involved in aminoglycoside recognition and extrusion by MexY have not been elucidated. Here, we report the cryo-electron microscopy structure of MexY to a resolution of 3.63 Å. The structure directly indicates two plausible pathways for drug export. It also suggests that MexY is capable of picking up antibiotics via the ceiling of the central cavity formed by the MexY trimer. Molecular dynamics simulations depict that MexY is able to use a tunnel connecting the central cavity to the funnel of the trimer to export its substrates.
IMPORTANCE: Here, we report the cryo-electron microscopy structure of the MexY multidrug efflux pump, posing the possibility that this pump is capable of capturing antibiotics from both the central cavity and the periplasmic cleft of the pump. The results indicate that MexY may utilize charged residues to bind and export drugs, mediating resistance to these antibiotics.
External linksmBio / PubMed:40042268 / PubMed Central
MethodsEM (single particle)
Resolution3.63 Å
Structure data

47796

9e9f

EMDB-47796, PDB-9e9f:
Pseudomonas aeruginosa MexY
Method: EM (single particle) / Resolution: 3.63 Å

Source
  • pseudomonas aeruginosa (bacteria)
KeywordsMEMBRANE PROTEIN / Pseudomonsa aeruginosa / MexY / RND / Efflux

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