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Structure paper

TitleUnique mechanisms to increase structural stability and enhance antigen binding in nanobodies.
Journal, issue, pagesStructure, Vol. 33, Page 677-690.e5, Year 2025
Publish dateJan 31, 2023 (structure data deposition date)
AuthorsKetaren, N.E. / Fridy, P.C. / Malashkevich, V. / Sanyal, T. / Brillantes, M. / Thompson, M.K. / Oren, D.A. / Bonanno, J.B. / Sali, A. / Almo, S.C. ...Ketaren, N.E. / Fridy, P.C. / Malashkevich, V. / Sanyal, T. / Brillantes, M. / Thompson, M.K. / Oren, D.A. / Bonanno, J.B. / Sali, A. / Almo, S.C. / Chait, B.T. / Rout, M.P.
External linksStructure / PubMed:39938509
MethodsX-ray diffraction
Resolution1.83 - 3.11 Å
Structure data

PDB-8g0i:
High Affinity nanobodies against GFP
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-8sfs:
High Affinity nanobodies against GFP
Method: X-RAY DIFFRACTION / Resolution: 2.37 Å

PDB-8sfv:
High affinity nanobodies to GFP
Method: X-RAY DIFFRACTION / Resolution: 1.83 Å

PDB-8sfx:
High Affinity nanobodies against GFP
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

PDB-8sfz:
High Affinity nanobodies against GFP
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-8sg3:
High Affinity nanobodies against GFP
Method: X-RAY DIFFRACTION / Resolution: 3.11 Å

PDB-8slc:
High Affinity nanobodies against GFP
Method: X-RAY DIFFRACTION / Resolution: 2.97 Å

Chemicals

ChemComp-CL:
Unknown entry

ChemComp-NA:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-NH4:
AMMONIUM ION

ChemComp-GOL:
GLYCEROL

ChemComp-SO4:
SULFATE ION

ChemComp-MLT:
D-MALATE

ChemComp-PO4:
PHOSPHATE ION

ChemComp-K:
Unknown entry

Source
  • aequorea victoria (jellyfish)
  • lama glama (llama)
KeywordsIMMUNE SYSTEM / Nanobody / nanobodies / GFP / green fluorescent protein / high-affinity antibody variant / antibody variant / single-domain antibody

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