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TitleStructures of a T1-like siphophage reveal capsid stabilization mechanisms and high structural similarities with a myophage.
Journal, issue, pagesStructure, Vol. 33, Issue 4, Page 663-676.e2, Year 2025
Publish dateApr 3, 2025
AuthorsCan Cai / Yueting Wang / Yunshu Liu / Qianqian Shao / Aohan Wang / Lin Li / Yaqi Zheng / Tianyi Zhang / Ziwen Luo / Chongguang Yang / Qianglin Fang /
PubMed AbstractBacteriophage T1, a member of Siphoviruses, infects Escherichia coli with high efficiency, making it a promising candidate for phage therapy. Here, we report the near-atomic structures of FCWL1, a T1- ...Bacteriophage T1, a member of Siphoviruses, infects Escherichia coli with high efficiency, making it a promising candidate for phage therapy. Here, we report the near-atomic structures of FCWL1, a T1-like phage that belongs to the T1 phage family. We focus on the head, the head-to-tail interface, and its surrounding components. The hexameric capsomer displays unique gaps between neighboring A domains of the major capsid proteins. These gaps are partially filled by the N-loop of the decoration protein, which adopts a unique conformation. These structural features suggest that the phage might employ a novel strategy for stabilizing its head. Furthermore, despite being a siphophage, the head and head-to-tail connector of the phage show high structural similarity to those of a myophage. These findings enhance our understanding of the structure, capsid stabilization mechanism, and evolution of phages in the T1 family.
External linksStructure / PubMed:39914381
MethodsEM (single particle)
Resolution3.1 - 3.79 Å
Structure data

61588

9jlf

EMDB-61588, PDB-9jlf:
Cryo-EM Structure of Bacteriophage FCWL1 head-to-tail interface
Method: EM (single particle) / Resolution: 3.3 Å

62429

9kmg

EMDB-62429, PDB-9kmg:
Cryo-EM Structure of Bacteriophage FCWL1 Capsid
Method: EM (single particle) / Resolution: 3.1 Å

EMDB-62430: The Asymmetric Reconstruction of the Portal Vertex of Phage FCWL1 Before Multibody Refinement
Method: EM (single particle) / Resolution: 3.54 Å

EMDB-62431: The Capsid Body in the Asymmetric Reconstruction of the Portal Vertex of Bacteriophage FCWL1
Method: EM (single particle) / Resolution: 3.48 Å

EMDB-62432: The Head-to-Tail interface body in the Asymmetric Reconstruction of the Portal Vertex of Bacteriophage FCWL1
Method: EM (single particle) / Resolution: 3.79 Å

62433

9kmh

EMDB-62433, PDB-9kmh:
The Composite Cryo-EM Structure of the Portal Vertex of Bacteriophage FCWL1
Method: EM (single particle) / Resolution: 3.5 Å

Source
  • escherichia phage fcwl1 (virus)
KeywordsVIRAL PROTEIN / T1-like phage / neck / tail / VIRUS / capsid / icosahedrally averaged

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