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TitleMolecular mechanism of ligand recognition and activation of lysophosphatidic acid receptor LPAR6.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 122, Issue 4, Page e2415426122, Year 2025
Publish dateJan 28, 2025
AuthorsYaning Duan / Zhenmei Xu / Boyu Hao / Anqi Zhang / Changyou Guo / Yuanzheng He /
PubMed AbstractLysophosphatidic acid (LPA) exerts its physiological roles through the endothelialdifferentiation gene (EDG) family LPA receptors (LPAR1-3) or the non-EDG family LPA receptors (LPAR4-6). LPAR6 plays ...Lysophosphatidic acid (LPA) exerts its physiological roles through the endothelialdifferentiation gene (EDG) family LPA receptors (LPAR1-3) or the non-EDG family LPA receptors (LPAR4-6). LPAR6 plays crucial roles in hair loss and cancer progression, yet its structural information is very limited. Here, we report the cryoelectron microscopy structure of LPA-bound human LPAR6 in complex with a mini G or G protein. These structures reveal a distinct ligand binding and recognition mode that differs significantly from that of LPAR1. Specifically, LPA uses its charged head to form an extensive polar interaction network with key polar residues on the extracellular side of transmembrane helix 5-6 and the extracellular loop 2. Structural comparisons and homology analysis suggest that the EDG and non-EDG families use two distinct modes for LPA binding. The structural observations are validated through functional mutagenesis studies. We further uncover the mechanisms of LPAR6 activation and principles of G-protein coupling. The structural information revealed by our study lays the groundwork for understanding LPAR6 signaling and provides a rational basis for designing compounds targeting LPAR6.
External linksProc Natl Acad Sci U S A / PubMed:39847322 / PubMed Central
MethodsEM (single particle)
Resolution2.89 - 3.06 Å
Structure data

60857

9itb

EMDB-60857, PDB-9itb:
LPA-bound LPAR6 in complex with miniGq
Method: EM (single particle) / Resolution: 2.89 Å

60859

9ite

EMDB-60859, PDB-9ite:
LPA-bound LPAR6 in complex with miniG13
Method: EM (single particle) / Resolution: 3.06 Å

Chemicals

ChemComp-NKP:
(2R)-2-hydroxy-3-(phosphonooxy)propyl (9E)-octadec-9-enoate

ChemComp-CLR:
CHOLESTEROL

ChemComp-PLM:
PALMITIC ACID

Source
  • homo sapiens (human)
  • lama glama (llama)
KeywordsMEMBRANE PROTEIN / GPCR-G-protein complex

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